Fluid shear regulates the kinetics and molecular mechanisms of activation-dependent platelet binding to colon carcinoma cells

Owen McCarty, Sameer Jadhav, Monica M. Burdick, William R. Bell, Konstantinos Konstantopoulos

Research output: Contribution to journalArticle

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Abstract

This study was undertaken to investigate the kinetics and molecular requirements of platelet binding to tumor cells in bulk suspensions subjected to a uniform linear shear field, using a human colon adenocarcinoma cell line (LS174T) as a model. The effects of shear rate (20-1000 s-1), shear exposure time (30-300 s), shear stress (at constant shear rate by adjusting the viscosity of the medium from 1.3-2.6 cP), cell concentration, and platelet activation on platelet-LS174T heteroaggregation were assessed. The results indicate that hydrodynamic shear-induced collisions augment platelet-LS174T binding, which is further potentiated by thrombin/GPRP-NH2. Peak adhesion efficiency occurs at low shear and decreases with increasing shear. Intercellular contact duration is the predominant factor limiting heteroaggregation at shear rates up to 200 s-1, whereas these interactions become shear stress-sensitive at ≥400 s-1. Heteroaggregation increases with platelet concentration due to an elevation of the intercellular collision frequency, whereas adhesion efficiency remains nearly constant. Moreover, hydrodynamic shear affects the receptor specificity of activation-dependent platelet binding to LS174T cells, as evidenced by the transition from a P-selectin-independent/Arg-Gly-Asp (RGD)-dependent process at 100 s-1 to a P-selectin/ αIIbβ3-dependent interaction at 800 s-1. This study demonstrates that platelet activation and a fluid-mechanical environment representative of the vasculature affect platelet-tumor cell adhesive interactions pertinent to the process of blood-borne metastasis.

Original languageEnglish (US)
Pages (from-to)836-848
Number of pages13
JournalBiophysical Journal
Volume83
Issue number2
StatePublished - 2002
Externally publishedYes

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Platelet Activation
Colon
Blood Platelets
Carcinoma
P-Selectin
Hydrodynamics
Viscosity
Thrombin
Cell Communication
Adhesives
Neoplasms
Suspensions
Adenocarcinoma
Neoplasm Metastasis
Cell Line

ASJC Scopus subject areas

  • Biophysics

Cite this

Fluid shear regulates the kinetics and molecular mechanisms of activation-dependent platelet binding to colon carcinoma cells. / McCarty, Owen; Jadhav, Sameer; Burdick, Monica M.; Bell, William R.; Konstantopoulos, Konstantinos.

In: Biophysical Journal, Vol. 83, No. 2, 2002, p. 836-848.

Research output: Contribution to journalArticle

McCarty, Owen ; Jadhav, Sameer ; Burdick, Monica M. ; Bell, William R. ; Konstantopoulos, Konstantinos. / Fluid shear regulates the kinetics and molecular mechanisms of activation-dependent platelet binding to colon carcinoma cells. In: Biophysical Journal. 2002 ; Vol. 83, No. 2. pp. 836-848.
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