Flt3-ligand as a vaccine adjuvant

Results in a study of Flt3-ligand plus tetanus toxoid immunization

Stephen (Steve) Chui, Timothy M. Clay, Paul J. Mosca, Amy C. Hobeika, Takuya Osada, Laurent Galibert, Dania Caron, H. Kim Lyerly, Michael A. Morse

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Dendritic cells (DC) efficiently process and present antigens to the effector arm of the immune system, thereby stimulating immunity against antigens of both foreign and self origin. Administration of Flt3-ligand (FL) has been reported to increase dendritic cell (DC) numbers in mice and humans. As a result, FL has attracted interest as an adjuvant for vaccine immunotherapy. To investigate whether FL might increase the immune response to a model recall antigen, we administered FL 25 μg/kg/d subcutaneously to six healthy volunteers followed by a standard injection of intramuscular tetanus toxoid (TT). A control cohort of six healthy volunteers received tetanus toxoid alone. Compared to subjects who received only TT, subjects who received Flt3L and TT had greater TT-specific DTH reactivity. In contrast, FL did not augment peripheral blood mononuclear cell proliferative responses or antibody responses to TT. FL resulted in inconsistent TT-specific T cell responses as measured by interferon-gamma ELISPOT and cytokine flow cytometry. We conclude that while FL mobilization of DC may enhance in vivo immune responses to a known immunogenic recall antigen, there are inconsistent effects on immune response detected by in vitro assays. Further study will be required to determine which individuals might experience augmentation of the immune response with FL.

Original languageEnglish (US)
Pages (from-to)536-549
Number of pages14
JournalJournal of Applied Research
Volume4
Issue number4
StatePublished - 2004
Externally publishedYes

Fingerprint

Tetanus Toxoid
Immunization
Vaccines
Dendritic Cells
Antigens
Healthy Volunteers
Enzyme-Linked Immunospot Assay
flt3 ligand protein
Intramuscular Injections
Immunotherapy
Interferon-gamma
Antibody Formation
Immune System
Immunity
Blood Cells
Flow Cytometry
Cell Count
Cytokines
T-Lymphocytes

Keywords

  • Flt3-ligand
  • Interferon gamma
  • T cell
  • Tetanus toxoid
  • Vaccination

ASJC Scopus subject areas

  • Medicine(all)
  • Pharmacology

Cite this

Chui, S. S., Clay, T. M., Mosca, P. J., Hobeika, A. C., Osada, T., Galibert, L., ... Morse, M. A. (2004). Flt3-ligand as a vaccine adjuvant: Results in a study of Flt3-ligand plus tetanus toxoid immunization. Journal of Applied Research, 4(4), 536-549.

Flt3-ligand as a vaccine adjuvant : Results in a study of Flt3-ligand plus tetanus toxoid immunization. / Chui, Stephen (Steve); Clay, Timothy M.; Mosca, Paul J.; Hobeika, Amy C.; Osada, Takuya; Galibert, Laurent; Caron, Dania; Lyerly, H. Kim; Morse, Michael A.

In: Journal of Applied Research, Vol. 4, No. 4, 2004, p. 536-549.

Research output: Contribution to journalArticle

Chui, SS, Clay, TM, Mosca, PJ, Hobeika, AC, Osada, T, Galibert, L, Caron, D, Lyerly, HK & Morse, MA 2004, 'Flt3-ligand as a vaccine adjuvant: Results in a study of Flt3-ligand plus tetanus toxoid immunization', Journal of Applied Research, vol. 4, no. 4, pp. 536-549.
Chui SS, Clay TM, Mosca PJ, Hobeika AC, Osada T, Galibert L et al. Flt3-ligand as a vaccine adjuvant: Results in a study of Flt3-ligand plus tetanus toxoid immunization. Journal of Applied Research. 2004;4(4):536-549.
Chui, Stephen (Steve) ; Clay, Timothy M. ; Mosca, Paul J. ; Hobeika, Amy C. ; Osada, Takuya ; Galibert, Laurent ; Caron, Dania ; Lyerly, H. Kim ; Morse, Michael A. / Flt3-ligand as a vaccine adjuvant : Results in a study of Flt3-ligand plus tetanus toxoid immunization. In: Journal of Applied Research. 2004 ; Vol. 4, No. 4. pp. 536-549.
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