Flow microfluorometric analysis of sperm DNA content

effect of cell shape on the fluorescence distribution

B. L. Gledhill, S. Lake, L. L. Steinmetz, Joe Gray, Crawford, P. N. Dean, M. A. Van Dilla

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

The DNA content of individual sperm from populations of acriflavine stained cells was investigated by analysis of fluorescence frequency distributions obtained with high resolution flow systems instruments. Sperm with spherical or cylindrical heads from three mollusk species produce narrow, symmetric fluorescence distributions. Flat sperm heads from six eutherian species produce asymmetric distributions consisting of a peak with a lateral extension to higher fluorescence values. The unexpected shape of these distributions was shown to be due to the flat geometry and high refractive index of the sperm heads in conjunction with the orthogonal axes of flow, excitation, and detection in the flow systems instruments. The theoretical and experimental results indicate that the lateral extension can be eliminated either by controlling the sperm orientation with planar flow conditions or by accounting for sperm orientation by means of orientation sensing.

Original languageEnglish (US)
Pages (from-to)367-375
Number of pages9
JournalJournal of Cellular Physiology
Volume87
Issue number3
DOIs
StatePublished - 1976
Externally publishedYes

Fingerprint

Cell Shape
Spermatozoa
Fluorescence
Sperm Head
DNA
Acriflavine
Refractometry
Mollusca
Refractive index
Geometry
Head
Population

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Flow microfluorometric analysis of sperm DNA content : effect of cell shape on the fluorescence distribution. / Gledhill, B. L.; Lake, S.; Steinmetz, L. L.; Gray, Joe; Crawford; Dean, P. N.; Van Dilla, M. A.

In: Journal of Cellular Physiology, Vol. 87, No. 3, 1976, p. 367-375.

Research output: Contribution to journalArticle

Gledhill, B. L. ; Lake, S. ; Steinmetz, L. L. ; Gray, Joe ; Crawford ; Dean, P. N. ; Van Dilla, M. A. / Flow microfluorometric analysis of sperm DNA content : effect of cell shape on the fluorescence distribution. In: Journal of Cellular Physiology. 1976 ; Vol. 87, No. 3. pp. 367-375.
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