Flow microfluorometric analysis of sperm DNA content: Effect of cell shape on the fluorescence distribution

Barton L. Gledhill, Suzanne Lake, Lloyd L. Steinmetz, Joe W. Gray, James R. Crawford, Phillip N. Dean, Marvin A. Van Dilla

Research output: Contribution to journalArticle

72 Scopus citations

Abstract

The DNA content of individual sperm from populations of acriflavine‐stained cells was investigated by analysis of fluorescence frequency distributions obtained with high‐resolution flow‐systems instruments. Sperm with spherical or cylindrical heads from three mollusk species produce narrow, symmetric fluorescence distributions. Flat sperm heads from six eutherian species produce asymmetric distributions consisting of a peak with a lateral extension to higher fluorescence values. The unexpected shape of these distributions was shown to be due to the flat geometry and high refractive index of the sperm heads in conjunction with the orthogonal axes of flow, excitation, and detection in the flow‐systems instruments. The theoretical and experimental results indicate that the lateral extension can be eliminated either by controlling the sperm orientation with planar flow conditions or by accounting for sperm orientation by means of orientation sensing.

Original languageEnglish (US)
Pages (from-to)367-375
Number of pages9
JournalJournal of Cellular Physiology
Volume87
Issue number3
DOIs
StatePublished - Mar 1976

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Fingerprint Dive into the research topics of 'Flow microfluorometric analysis of sperm DNA content: Effect of cell shape on the fluorescence distribution'. Together they form a unique fingerprint.

  • Cite this