Flow infusion electrospray ionization high-resolution mass spectrometry of the chloride adducts of sphingolipids, glycerophospholipids, glycerols, hydroxy fatty acids, fatty acid esters of hydroxy fatty acids (FAHFA), and modified nucleosides

Paul L. Wood, Randall L. Woltjer

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Ceramides are generally monitored via either their anions or cations formed with electrospray ionization (ESI). While these methods appear adequate for chromatographic assays, they offer limited sensitivity with flow infusion ESI (“shotgun lipidomics”). We therefore evaluated the chloride adducts [M + Cl] of a number of lipid classes and found 10- to 50-fold increases in assay sensitivity for ceramides, hexosylceramides, lactosylceramides, phytoceramides, dihydroceramides, and ceramide phosphoethanolamines. Chloride adducts of glycerophosphocholines, sphingomyelins, hydroxyl fatty acids, and modified nucleotides also were characterized. Chloride adducts of glycerophosphocholines were particularly useful for the MS/MS analysis of the constituent fatty acids at sn-1 and sn-2.

Original languageEnglish (US)
Title of host publicationNeuromethods
PublisherHumana Press Inc.
Pages69-76
Number of pages8
DOIs
StatePublished - 2021

Publication series

NameNeuromethods
Volume159
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

Keywords

  • Ceramides
  • Chloride adducts
  • Negative ion electrospray mass spectrometry

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Psychiatry and Mental health

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