Flow cytometric analysis of deoxyribonucleic acid in human granulosa cells as a function of chronological age and ovulation induction regimen

David Seifer, Julie Honig, Alan S. Penzias, Gad Lavy, Prakash M. Nadkarni, Ervin E. Jones, Alan H. Decherney, Stuart D. Flynn

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We examined whether the proliferative index of granulosa cells as determined by flow cytometry varied with a woman's age or ovulation induction regimen that included leuprolide acetate (LA). This prospective cohort study included three groups of patients undergoing assisted reproductive technologies. Group I consisted of 9 women age less than or equal to 30 yr, who received LA plus human menopausal gonadotropin (hMG). Group II included 9 women age more than or equal to 40 yr, who received LA plus hMG. Group III consisted of 6 women age less than or equal to 30 yr who received hMG alone. A total of 79 preovulatory follicles containing greater than 104 granulosa cells were obtained from these 24 women and examined by flow cytometry. Group I was compared to group II to match for ovulation induction regimen and to examine proliferative index as a function of age. Group I was compared to group III to match for age and to examine proliferative index as a function of ovulation induction regimen. Outcome measures included proliferative index of granulosa cells as a function of age, ovulation induction regimen, ampules of hMG, estradiol on day of hCG, and serum FSH. Group I demonstrated a greater proliferative index than group II: 23.4% ± 1.4 vs. 18.4% ± 0.96 (P <0.01). Group I had a greater proliferative index than group III: 23.4% ± 1.4 vs. 11.9 ± 0.61 (P <0.001). Although both age and the presence of LA appeared to affect the PI, multiple linear regression demonstrated that only the addition of LA and not age, per se, had an independent effect upon granulosa cells undergoing proliferation (P <0.0005). We conclude that LA followed by hMG leads to an increase in the percentage of granulosa cells undergoing proliferation when compared to ovulation induction regimens that include hMG alone. Chronological age does not appear to have a significant independent influence upon the proliferative index.

Original languageEnglish (US)
Pages (from-to)636-640
Number of pages5
JournalJournal of Clinical Endocrinology and Metabolism
Volume75
Issue number2
StatePublished - Aug 1992
Externally publishedYes

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Leuprolide
Menotropins
Ovulation Induction
Granulosa Cells
DNA
Flow cytometry
Cell proliferation
Flow Cytometry
Cell Proliferation
Assisted Reproductive Techniques
Linear regression
Estradiol
Linear Models
Cohort Studies
Outcome Assessment (Health Care)
Prospective Studies
Serum

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Flow cytometric analysis of deoxyribonucleic acid in human granulosa cells as a function of chronological age and ovulation induction regimen. / Seifer, David; Honig, Julie; Penzias, Alan S.; Lavy, Gad; Nadkarni, Prakash M.; Jones, Ervin E.; Decherney, Alan H.; Flynn, Stuart D.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 75, No. 2, 08.1992, p. 636-640.

Research output: Contribution to journalArticle

Seifer, D, Honig, J, Penzias, AS, Lavy, G, Nadkarni, PM, Jones, EE, Decherney, AH & Flynn, SD 1992, 'Flow cytometric analysis of deoxyribonucleic acid in human granulosa cells as a function of chronological age and ovulation induction regimen', Journal of Clinical Endocrinology and Metabolism, vol. 75, no. 2, pp. 636-640.
Seifer, David ; Honig, Julie ; Penzias, Alan S. ; Lavy, Gad ; Nadkarni, Prakash M. ; Jones, Ervin E. ; Decherney, Alan H. ; Flynn, Stuart D. / Flow cytometric analysis of deoxyribonucleic acid in human granulosa cells as a function of chronological age and ovulation induction regimen. In: Journal of Clinical Endocrinology and Metabolism. 1992 ; Vol. 75, No. 2. pp. 636-640.
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abstract = "We examined whether the proliferative index of granulosa cells as determined by flow cytometry varied with a woman's age or ovulation induction regimen that included leuprolide acetate (LA). This prospective cohort study included three groups of patients undergoing assisted reproductive technologies. Group I consisted of 9 women age less than or equal to 30 yr, who received LA plus human menopausal gonadotropin (hMG). Group II included 9 women age more than or equal to 40 yr, who received LA plus hMG. Group III consisted of 6 women age less than or equal to 30 yr who received hMG alone. A total of 79 preovulatory follicles containing greater than 104 granulosa cells were obtained from these 24 women and examined by flow cytometry. Group I was compared to group II to match for ovulation induction regimen and to examine proliferative index as a function of age. Group I was compared to group III to match for age and to examine proliferative index as a function of ovulation induction regimen. Outcome measures included proliferative index of granulosa cells as a function of age, ovulation induction regimen, ampules of hMG, estradiol on day of hCG, and serum FSH. Group I demonstrated a greater proliferative index than group II: 23.4{\%} ± 1.4 vs. 18.4{\%} ± 0.96 (P <0.01). Group I had a greater proliferative index than group III: 23.4{\%} ± 1.4 vs. 11.9 ± 0.61 (P <0.001). Although both age and the presence of LA appeared to affect the PI, multiple linear regression demonstrated that only the addition of LA and not age, per se, had an independent effect upon granulosa cells undergoing proliferation (P <0.0005). We conclude that LA followed by hMG leads to an increase in the percentage of granulosa cells undergoing proliferation when compared to ovulation induction regimens that include hMG alone. Chronological age does not appear to have a significant independent influence upon the proliferative index.",
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AU - Decherney, Alan H.

AU - Flynn, Stuart D.

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