FIFTH INTERNATIONAL CONGRESS OF HUMAN GENETICS

A. V. Carrano, Joe Gray, R. L. Balhorn, D. H. Moore

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Metaphase chromosomes isolated from Chinese hamster, Indian muntjac and human cells are stained with ethidium bromide and measured for DNA content by flow cytometry at rates of 105/min. The result, a histogram of chromosome frequency versus DNA amount, constitutes a flow karyotype. The chromosomes from a near diploid Chinese hamster cell line (M3-1) with 14 distinct chromosome types yields a histogram with 9 peaks. This is in agreement with scanning cytophotometric measurements of DNA in the same chromosomes. Each of the 5 different chromosomes isolated from male Indian muntjac cells produce a unique peak in the flow karyotype while the 24 human chromosome types are distributed into 7 broad peaks according to their DNA amounts. The time for data collection and reduction is about 10 min per karyotype. In addition, flow sorting permits separation and collection of highly purified populations of single chromosome types for cytological or biochemical analysis. This new flow cytogenetics technology has excellent potential for rapid monitoring of human populations, aberration detection and biochemical analysis of DNA or protein on a per chromosome basis.

Original languageEnglish (US)
Title of host publicationEXCERPTA MED. AMSTERDAM, I.C.S.
VolumeNo. 397
StatePublished - 1976
Externally publishedYes

Fingerprint

Medical Genetics
Chromosomes
Muntjacs
Karyotype
DNA
Cricetulus
Chromosomes, Human, Pair 14
Chromosomes, Human, Pair 5
Ethidium
Human Chromosomes
Metaphase
Diploidy
Cytogenetics
Population
Flow Cytometry
Technology
Cell Line
Proteins

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Carrano, A. V., Gray, J., Balhorn, R. L., & Moore, D. H. (1976). FIFTH INTERNATIONAL CONGRESS OF HUMAN GENETICS. In EXCERPTA MED. AMSTERDAM, I.C.S. (Vol. No. 397)

FIFTH INTERNATIONAL CONGRESS OF HUMAN GENETICS. / Carrano, A. V.; Gray, Joe; Balhorn, R. L.; Moore, D. H.

EXCERPTA MED. AMSTERDAM, I.C.S.. Vol. No. 397 1976.

Research output: Chapter in Book/Report/Conference proceedingChapter

Carrano, AV, Gray, J, Balhorn, RL & Moore, DH 1976, FIFTH INTERNATIONAL CONGRESS OF HUMAN GENETICS. in EXCERPTA MED. AMSTERDAM, I.C.S.. vol. No. 397.
Carrano AV, Gray J, Balhorn RL, Moore DH. FIFTH INTERNATIONAL CONGRESS OF HUMAN GENETICS. In EXCERPTA MED. AMSTERDAM, I.C.S.. Vol. No. 397. 1976
Carrano, A. V. ; Gray, Joe ; Balhorn, R. L. ; Moore, D. H. / FIFTH INTERNATIONAL CONGRESS OF HUMAN GENETICS. EXCERPTA MED. AMSTERDAM, I.C.S.. Vol. No. 397 1976.
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