Fibroblast Growth Factor Receptor 3 Induces Gene Expression Primarily through Ras-independent Signal Transduction Pathways

Deog Young Choi, Juan Jose Toledo-Aral, Hsien Y. Lin, Irene Ischenko, Lillian Medina, Patrick Safo, Gail Mandel, S. Rock Levinson, Simon Halegoua, Michael J. Hayman

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Fibroblast growth factor receptors (FGFR) are widely expressed in many tissues and cell types, and the temporal expression of these receptors and their ligands play important roles in the control of development. There are four FGFR family members, FGFR-1-4, and understanding the ability of these receptors to transduce signals is central to understanding how they function in controlling differentiation and development. We have utilized signal transduction by FGF-1 in PC12 cells to compare the ability of FGFR-1 and FGFR-3 to elicit the neuronal phenotype. In PC12 cells FGFR-1 is much more potent in the induction of neurite outgrowth than FGFR-3. This correlated with the ability of FGFR-1 to induce robust and sustained activation of the Ras-dependent mitogen-activated protein kinase pathways. In contrast, FGFR-3 could not induce strong sustained Ras-dependent signals. In this study, we analyzed the ability of FGFR-3 to induce the expression of sodium channels, peripherin, and Thy-1 in PC12 cells because all three of these proteins are known to be induced via Ras-independent pathways. We determined that FGFR-3 was capable of inducing several Ras-independent gene expression pathways important to the neuronal phenotype to a level equivalent of that induced by FGFR-1. Thus, FGFR-3 elicits phenotypic changes primarily though activation of Ras-independent pathways in the absence of robust Ras-dependent signals.

Original languageEnglish (US)
Pages (from-to)5116-5122
Number of pages7
JournalJournal of Biological Chemistry
Volume276
Issue number7
DOIs
StatePublished - Feb 16 2001
Externally publishedYes

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Receptor, Fibroblast Growth Factor, Type 3
Signal transduction
Receptor, Fibroblast Growth Factor, Type 1
Gene expression
Signal Transduction
Aptitude
Gene Expression
PC12 Cells
Fibroblast Growth Factor Receptors
Receptor, Fibroblast Growth Factor, Type 4
Peripherins
Chemical activation
Phenotype
Fibroblast Growth Factor 1
ras Genes
Sodium Channels
Mitogen-Activated Protein Kinases
Tissue
Ligands

ASJC Scopus subject areas

  • Biochemistry

Cite this

Choi, D. Y., Toledo-Aral, J. J., Lin, H. Y., Ischenko, I., Medina, L., Safo, P., ... Hayman, M. J. (2001). Fibroblast Growth Factor Receptor 3 Induces Gene Expression Primarily through Ras-independent Signal Transduction Pathways. Journal of Biological Chemistry, 276(7), 5116-5122. https://doi.org/10.1074/jbc.M002959200

Fibroblast Growth Factor Receptor 3 Induces Gene Expression Primarily through Ras-independent Signal Transduction Pathways. / Choi, Deog Young; Toledo-Aral, Juan Jose; Lin, Hsien Y.; Ischenko, Irene; Medina, Lillian; Safo, Patrick; Mandel, Gail; Levinson, S. Rock; Halegoua, Simon; Hayman, Michael J.

In: Journal of Biological Chemistry, Vol. 276, No. 7, 16.02.2001, p. 5116-5122.

Research output: Contribution to journalArticle

Choi, DY, Toledo-Aral, JJ, Lin, HY, Ischenko, I, Medina, L, Safo, P, Mandel, G, Levinson, SR, Halegoua, S & Hayman, MJ 2001, 'Fibroblast Growth Factor Receptor 3 Induces Gene Expression Primarily through Ras-independent Signal Transduction Pathways', Journal of Biological Chemistry, vol. 276, no. 7, pp. 5116-5122. https://doi.org/10.1074/jbc.M002959200
Choi, Deog Young ; Toledo-Aral, Juan Jose ; Lin, Hsien Y. ; Ischenko, Irene ; Medina, Lillian ; Safo, Patrick ; Mandel, Gail ; Levinson, S. Rock ; Halegoua, Simon ; Hayman, Michael J. / Fibroblast Growth Factor Receptor 3 Induces Gene Expression Primarily through Ras-independent Signal Transduction Pathways. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 7. pp. 5116-5122.
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