Fibrillin-1 and fibulin-2 interact and are colocalized in some tissues

Dieter P. Reinhardt, Takako Sasaki, Bette J. Dzamba, Douglas R. Keene, Mon Li Chu, Walter Göhring, Rupert Timpl, Lynn Y. Sakai

Research output: Contribution to journalArticle

176 Scopus citations

Abstract

Microfibrils 10-12 nm in diameter are found in elastic and non-elastic tissues with fibrillin as a major component. Little is known about the supramolecular structure of these microfibrils and the protein interactions it is based on. To identify protein binding ligands of fibrillin-1, we tested binding of recombinant fibrillin-1 peptides to different extracellular matrix proteins in solid phase assays. Among the proteins tested, only fibulin-2 showed significant binding to rF11, the N-terminal half of fibrillin-1, in a calcium-dependent manner. Surface plasmon resonance demonstrated high affinity binding with a K(d) = 56 nM. With overlapping recombinant fibrillin-1 peptides, the binding site for fibulin- 2 was narrowed down to the N terminus of fibrillin-1 (amino acid positions 45-450). Immunofluorescence in tissues demonstrated colocalization of fibrillin and fibulin-2 in skin, perichondrium, elastic intima of blood vessels, and kidney glomerulus. Fibulin-2 was not present in ocular ciliary zonules, tendon, and the connective tissue around kidney tubules and lung alveoli, which all contain fibrillin. Immunogold labeling of fibulin-2 on microfibrils in skin was found preferentially at the interface between microfibrils and the amorphous elastin core, suggesting that in vivo the interaction between fibrillin-1 and fibulin-2 is regulated by cellular expression and deposition as well as by protein-protein interactions.

Original languageEnglish (US)
Pages (from-to)19489-19496
Number of pages8
JournalJournal of Biological Chemistry
Volume271
Issue number32
DOIs
StatePublished - Aug 27 1996

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Reinhardt, D. P., Sasaki, T., Dzamba, B. J., Keene, D. R., Chu, M. L., Göhring, W., Timpl, R., & Sakai, L. Y. (1996). Fibrillin-1 and fibulin-2 interact and are colocalized in some tissues. Journal of Biological Chemistry, 271(32), 19489-19496. https://doi.org/10.1074/jbc.271.32.19489