Fibrillin-1 and fibulin-2 interact and are colocalized in some tissues

Dieter P. Reinhardt, Takako Sasaki, Bette J. Dzamba, Douglas R. Keene, Mon Li Chu, Walter Göhring, Rupert Timpl, Lynn Sakai

Research output: Contribution to journalArticle

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Abstract

Microfibrils 10-12 nm in diameter are found in elastic and non-elastic tissues with fibrillin as a major component. Little is known about the supramolecular structure of these microfibrils and the protein interactions it is based on. To identify protein binding ligands of fibrillin-1, we tested binding of recombinant fibrillin-1 peptides to different extracellular matrix proteins in solid phase assays. Among the proteins tested, only fibulin-2 showed significant binding to rF11, the N-terminal half of fibrillin-1, in a calcium-dependent manner. Surface plasmon resonance demonstrated high affinity binding with a K(d) = 56 nM. With overlapping recombinant fibrillin-1 peptides, the binding site for fibulin- 2 was narrowed down to the N terminus of fibrillin-1 (amino acid positions 45-450). Immunofluorescence in tissues demonstrated colocalization of fibrillin and fibulin-2 in skin, perichondrium, elastic intima of blood vessels, and kidney glomerulus. Fibulin-2 was not present in ocular ciliary zonules, tendon, and the connective tissue around kidney tubules and lung alveoli, which all contain fibrillin. Immunogold labeling of fibulin-2 on microfibrils in skin was found preferentially at the interface between microfibrils and the amorphous elastin core, suggesting that in vivo the interaction between fibrillin-1 and fibulin-2 is regulated by cellular expression and deposition as well as by protein-protein interactions.

Original languageEnglish (US)
Pages (from-to)19489-19496
Number of pages8
JournalJournal of Biological Chemistry
Volume271
Issue number32
DOIs
StatePublished - Aug 27 1996

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Microfibrils
Tissue
Skin
Proteins
Kidney Glomerulus
Kidney Tubules
Peptides
Surface Plasmon Resonance
Elastin
Extracellular Matrix Proteins
Tendons
Blood vessels
Surface plasmon resonance
Protein Binding
Connective Tissue
Labeling
Fluorescent Antibody Technique
Blood Vessels
Fibrillin-1
fibulin 2

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)
  • Molecular Biology
  • Cell Biology

Cite this

Reinhardt, D. P., Sasaki, T., Dzamba, B. J., Keene, D. R., Chu, M. L., Göhring, W., ... Sakai, L. (1996). Fibrillin-1 and fibulin-2 interact and are colocalized in some tissues. Journal of Biological Chemistry, 271(32), 19489-19496. https://doi.org/10.1074/jbc.271.32.19489

Fibrillin-1 and fibulin-2 interact and are colocalized in some tissues. / Reinhardt, Dieter P.; Sasaki, Takako; Dzamba, Bette J.; Keene, Douglas R.; Chu, Mon Li; Göhring, Walter; Timpl, Rupert; Sakai, Lynn.

In: Journal of Biological Chemistry, Vol. 271, No. 32, 27.08.1996, p. 19489-19496.

Research output: Contribution to journalArticle

Reinhardt, DP, Sasaki, T, Dzamba, BJ, Keene, DR, Chu, ML, Göhring, W, Timpl, R & Sakai, L 1996, 'Fibrillin-1 and fibulin-2 interact and are colocalized in some tissues', Journal of Biological Chemistry, vol. 271, no. 32, pp. 19489-19496. https://doi.org/10.1074/jbc.271.32.19489
Reinhardt DP, Sasaki T, Dzamba BJ, Keene DR, Chu ML, Göhring W et al. Fibrillin-1 and fibulin-2 interact and are colocalized in some tissues. Journal of Biological Chemistry. 1996 Aug 27;271(32):19489-19496. https://doi.org/10.1074/jbc.271.32.19489
Reinhardt, Dieter P. ; Sasaki, Takako ; Dzamba, Bette J. ; Keene, Douglas R. ; Chu, Mon Li ; Göhring, Walter ; Timpl, Rupert ; Sakai, Lynn. / Fibrillin-1 and fibulin-2 interact and are colocalized in some tissues. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 32. pp. 19489-19496.
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