Fiber-based combined optical coherence and multiphoton microscopy

Gangjun Liu, Zhongping Chen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

This manuscript demonstrates a multimodal imaging system which combined multiphoton microscopy (MPM) imaging modality with Fourier domain (FD) optical coherence microscopy (OCM) imaging modality. The system used a single fiber-based femtosecond laser as the light source for both MPM and OCM modality. The femtosecond fiber laser has a central wavlength of 1.03 μm, a pulse width of 120 fs and a bandwidth of 29 nm. The systems used fiber-based devices for both MPM and OCM imaging. The MPM and OCM shared the same excitation light path. The excitation light was delivered with the core of a dual-clad fiber. The MPM and OCM signal was collected by different parts of the dual-clad fiber. The MPM signal was collected by the clad of the dual-clad fiber and the OCM signal was collected by the core of the dual-clad fiber. The FD OCM used a home-built InGaAs detector array spectrometer with a maximum Aline speed of 7.7 KHz. The multiphoton signal collection efficiency was analyzed and several imaging modality including second harmonic generation imaging, two-photon excited fluorescence and optical coherent microscopy imaging were demonstrated.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7892
DOIs
StatePublished - 2011
Externally publishedYes
EventMultimodal Biomedical Imaging VI - San Francisco, CA, United States
Duration: Jan 22 2011Jan 23 2011

Other

OtherMultimodal Biomedical Imaging VI
CountryUnited States
CitySan Francisco, CA
Period1/22/111/23/11

Fingerprint

Microscopy
Microscopic examination
microscopy
fibers
Fibers
Imaging techniques
Light
Lasers
Multimodal Imaging
Optical Fibers
Harmonic generation
Fiber lasers
Ultrashort pulses
Photons
Imaging systems
excitation
fiber lasers
Light sources
Spectrometers
harmonic generations

Keywords

  • multimodal imaging
  • multiphoton microscopy
  • nonlinear laser scanning microscopy
  • optical coherence microscopy

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Liu, G., & Chen, Z. (2011). Fiber-based combined optical coherence and multiphoton microscopy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7892). [78920A] https://doi.org/10.1117/12.874267

Fiber-based combined optical coherence and multiphoton microscopy. / Liu, Gangjun; Chen, Zhongping.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7892 2011. 78920A.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Liu, G & Chen, Z 2011, Fiber-based combined optical coherence and multiphoton microscopy. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7892, 78920A, Multimodal Biomedical Imaging VI, San Francisco, CA, United States, 1/22/11. https://doi.org/10.1117/12.874267
Liu G, Chen Z. Fiber-based combined optical coherence and multiphoton microscopy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7892. 2011. 78920A https://doi.org/10.1117/12.874267
Liu, Gangjun ; Chen, Zhongping. / Fiber-based combined optical coherence and multiphoton microscopy. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7892 2011.
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