FGF signaling in chick lens development

Anh Chi N Le, Linda Musil

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

The prevailing concept has been that an FGF induces epithelial-to-fiber differentiation in the mammalian lens, whereas chick lens cells are unresponsive to FGF and are instead induced to differentiate by IGF/insulin-type factors. We show here that when treated for periods in excess of those used in previous investigations (>5 h), purified recombinant FGFs stimulate proliferation of primary cultures of embryonic chick lens epithelial cells and (at higher concentrations) expression of the fiber differentiation markers δ-crystallin and CP49. Surprisingly, upregulation of proliferation and δ-crystallin synthesis by FGF does not require activation of ERK kinases. ERK function is, however, essential for stimulation of δ-crystallin expression in response to insulin or IGF-1. Vitreous humor, the presumptive source of differentiation-promoting activity in vivo, contains a factor capable of diffusing out of the vitreous body and inducing δ-crystallin and CP49 expression in chick lens cultures. This factor binds heparin with high affinity and increases δ-crystallin expression in an ERK-insensitive manner, properties consistent with an FGF but not insulin or IGF. Our findings indicate that differentiation in the chick lens is likely to be mediated by an FGF and provide the first insights into the role of the ERK pathway in growth factor-induced signal transduction in the lens.

Original languageEnglish (US)
Pages (from-to)394-411
Number of pages18
JournalDevelopmental Biology
Volume233
Issue number2
DOIs
StatePublished - May 15 2001

Fingerprint

Crystallins
Lenses
Vitreous Body
Insulin
MAP Kinase Signaling System
Differentiation Antigens
Insulin-Like Growth Factor I
Heparin
Signal Transduction
Intercellular Signaling Peptides and Proteins
Phosphotransferases
Up-Regulation
Epithelial Cells

Keywords

  • Chick
  • Differentiation
  • ERK
  • FGF
  • IGF
  • Lens
  • MAP kinase
  • Vitreous humor

ASJC Scopus subject areas

  • Developmental Biology

Cite this

FGF signaling in chick lens development. / Le, Anh Chi N; Musil, Linda.

In: Developmental Biology, Vol. 233, No. 2, 15.05.2001, p. 394-411.

Research output: Contribution to journalArticle

Le, Anh Chi N ; Musil, Linda. / FGF signaling in chick lens development. In: Developmental Biology. 2001 ; Vol. 233, No. 2. pp. 394-411.
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