Fetal serum folate concentrations and placental folate transport in obese women

Margaret F. Carter, Theresa L. Powell, Cun Li, Leslie Myatt, Donald Dudley, Peter Nathanielsz, Thomas Jansson

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Objective: We hypothesized that fetal folate serum concentrations are lower and placental folate transport is impaired in pregnancies of obese women. Study Design: Umbilical vein serum and placental tissue were collected from normal weight and obese pregnant women at term. Cellular localization (immunohistochemistry) of folate receptor-α (FR-α), proton coupled folate transporter (PCFT), and reduced folate carrier (RFC) was established. Protein expression (Western blot) and transporter activity (isotope labeled methyltetrahydrofolate) were determined in syncytiotrophoblast microvillus membranes (MVM). Results: Fetal folate concentrations were similar in obese women as compared with normal weight women. Protein expression of FR-α in microvillus membranes was increased (+173%), in RFC was decreased (-41%), and in PCFT was unchanged. However, activity of FR-α, PCFT, and RFC was unaltered in obesity. Conclusion: Fetal serum folate concentrations and placental folate transport activity are not altered in obesity at term, which suggests that limited availability of folate does not contribute to abnormal gene methylation and developmental programming.

Original languageEnglish (US)
Pages (from-to)83.e17-83.e25
JournalAmerican journal of obstetrics and gynecology
Volume205
Issue number1
DOIs
StatePublished - Jul 2011

    Fingerprint

Keywords

  • methyl donor
  • obesity
  • pregnancy
  • trophoblast

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this