Fetal rabbit gastric epithelial cells cultured on floating collagen gels

C. D. Logsdon, C. A. Bisbee, Michael Rutten, T. E. Machen

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Cells were isolated from ~ 30 d fetal rabbit stomachs and cultured on floating collagen gels. Electron microscopy showed monolayers in which only one cell type persisted. These columnar cells were joined at apical borders by tight junctions and contained an extensive endoplasmic reticular network with an occasional intracellular canaliculus. They also occasionally contained what appeared to be secretory granules (mucus?), and therefore had some characteristics of all the cell types of the intact fetal stomachs, which showed oxyntic, mucous, and undifferentiated cells. In Ussing chambers with Ringer's solution on both sides, cultures developed transepithelial potential (potential difference [PD], mV, mucosa ground) = 13, resistance (resistance [B], Ω-cm2) = 285, an short-circuit current (I(sc), μA/cm2) = 45 (n=7), clearly indicating that cellular polarity and junctional integrity were maintained. These transport parameters were somewhat different for intact fetal stomachs (PD=20, R=70, and I(sc)=220 [n=4]), which may be due to extensive folding of intact fetal stomachs or the presence of only on cell type in culture, or both. Although gastric stimulants histamine, dibutyryl cycle AMP (dbcAMP), and isobutylmethylxanthine (IMX) (a phosphodiesterase inhibitor) did not elicit H+ secretion or electrophysiological changes in monolayers or intact stomachs, 10-4 M apical amiloride caused a decrease in I(sc) in cultured monolayers (27%) and intact stomachs (50%). Thus, Na+ transport seems to be a significant fraction of ion transport in both preparations. This culture system may allow the study of oxyntic cell differentiation and the development of H+, Na+, and Cl- transport in the gastric mucosa.

Original languageEnglish (US)
Pages (from-to)233-242
Number of pages10
JournalIn Vitro
Volume18
Issue number3 I
StatePublished - 1982
Externally publishedYes

Fingerprint

Monolayers
Stomach
Collagen
Gels
Epithelial Cells
Rabbits
Phosphodiesterase Inhibitors
Amiloride
Adenosine Monophosphate
Short circuit currents
Histamine
Electron microscopy
Ions
Gastric Parietal Cells
Tight Junctions
Ion Transport
Secretory Vesicles
Mucus
Gastric Mucosa
Cell Differentiation

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Developmental Biology

Cite this

Logsdon, C. D., Bisbee, C. A., Rutten, M., & Machen, T. E. (1982). Fetal rabbit gastric epithelial cells cultured on floating collagen gels. In Vitro, 18(3 I), 233-242.

Fetal rabbit gastric epithelial cells cultured on floating collagen gels. / Logsdon, C. D.; Bisbee, C. A.; Rutten, Michael; Machen, T. E.

In: In Vitro, Vol. 18, No. 3 I, 1982, p. 233-242.

Research output: Contribution to journalArticle

Logsdon, CD, Bisbee, CA, Rutten, M & Machen, TE 1982, 'Fetal rabbit gastric epithelial cells cultured on floating collagen gels', In Vitro, vol. 18, no. 3 I, pp. 233-242.
Logsdon CD, Bisbee CA, Rutten M, Machen TE. Fetal rabbit gastric epithelial cells cultured on floating collagen gels. In Vitro. 1982;18(3 I):233-242.
Logsdon, C. D. ; Bisbee, C. A. ; Rutten, Michael ; Machen, T. E. / Fetal rabbit gastric epithelial cells cultured on floating collagen gels. In: In Vitro. 1982 ; Vol. 18, No. 3 I. pp. 233-242.
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