Fetal bovine serum inhibits chondrocyte collagenase production: Interleukin 1 reverses this effect

Rita A. Kandel, Kenneth P.H. Pritzker, Gordon B. Mills, Tony F. Cruz

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Bovine articular chondrocytes incubated in medium which was serum free or contained low levels of fetal bovine serum (less than 5%) constitutively produced collagenase. Increasing the concentration of serum in the culture medium inhibited the production of collagenase. Addition of interleukin 1 and lipopolysaccharide reversed the inhibitory effect of serum. Phorbol esters only stimulated collagenase production when the serum concentration was at least 10%. These data suggest that there is a factor(s) in fetal bovine serum that inhibits collagenase production by chondrocytes and this can be reversed by agents such as interleukin 1.

Original languageEnglish (US)
Pages (from-to)130-134
Number of pages5
JournalBBA - Molecular Cell Research
Volume1053
Issue number2-3
DOIs
StatePublished - Jul 12 1990
Externally publishedYes

Keywords

  • Chondrocyte
  • Collagenase
  • Fetal bovine serum
  • Interleukin 1

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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