Abstract
Bovine articular chondrocytes incubated in medium which was serum free or contained low levels of fetal bovine serum (less than 5%) constitutively produced collagenase. Increasing the concentration of serum in the culture medium inhibited the production of collagenase. Addition of interleukin 1 and lipopolysaccharide reversed the inhibitory effect of serum. Phorbol esters only stimulated collagenase production when the serum concentration was at least 10%. These data suggest that there is a factor(s) in fetal bovine serum that inhibits collagenase production by chondrocytes and this can be reversed by agents such as interleukin 1.
Original language | English (US) |
---|---|
Pages (from-to) | 130-134 |
Number of pages | 5 |
Journal | BBA - Molecular Cell Research |
Volume | 1053 |
Issue number | 2-3 |
DOIs | |
State | Published - Jul 12 1990 |
Externally published | Yes |
Keywords
- Chondrocyte
- Collagenase
- Fetal bovine serum
- Interleukin 1
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology