Fetal bovine serum inhibits chondrocyte collagenase production

Interleukin 1 reverses this effect

Rita A. Kandel, Kenneth P H Pritzker, Gordon Mills, Tony F. Cruz

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Bovine articular chondrocytes incubated in medium which was serum free or contained low levels of fetal bovine serum (less than 5%) constitutively produced collagenase. Increasing the concentration of serum in the culture medium inhibited the production of collagenase. Addition of interleukin 1 and lipopolysaccharide reversed the inhibitory effect of serum. Phorbol esters only stimulated collagenase production when the serum concentration was at least 10%. These data suggest that there is a factor(s) in fetal bovine serum that inhibits collagenase production by chondrocytes and this can be reversed by agents such as interleukin 1.

Original languageEnglish (US)
Pages (from-to)130-134
Number of pages5
JournalBBA - Molecular Cell Research
Volume1053
Issue number2-3
DOIs
StatePublished - Jul 12 1990
Externally publishedYes

Fingerprint

Collagenases
Chondrocytes
Interleukin-1
Serum
Phorbol Esters
Culture Media
Lipopolysaccharides
Joints

Keywords

  • Chondrocyte
  • Collagenase
  • Fetal bovine serum
  • Interleukin 1

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Fetal bovine serum inhibits chondrocyte collagenase production : Interleukin 1 reverses this effect. / Kandel, Rita A.; Pritzker, Kenneth P H; Mills, Gordon; Cruz, Tony F.

In: BBA - Molecular Cell Research, Vol. 1053, No. 2-3, 12.07.1990, p. 130-134.

Research output: Contribution to journalArticle

Kandel, Rita A. ; Pritzker, Kenneth P H ; Mills, Gordon ; Cruz, Tony F. / Fetal bovine serum inhibits chondrocyte collagenase production : Interleukin 1 reverses this effect. In: BBA - Molecular Cell Research. 1990 ; Vol. 1053, No. 2-3. pp. 130-134.
@article{136a42dbf9bb4da69ea133db6f6b432d,
title = "Fetal bovine serum inhibits chondrocyte collagenase production: Interleukin 1 reverses this effect",
abstract = "Bovine articular chondrocytes incubated in medium which was serum free or contained low levels of fetal bovine serum (less than 5{\%}) constitutively produced collagenase. Increasing the concentration of serum in the culture medium inhibited the production of collagenase. Addition of interleukin 1 and lipopolysaccharide reversed the inhibitory effect of serum. Phorbol esters only stimulated collagenase production when the serum concentration was at least 10{\%}. These data suggest that there is a factor(s) in fetal bovine serum that inhibits collagenase production by chondrocytes and this can be reversed by agents such as interleukin 1.",
keywords = "Chondrocyte, Collagenase, Fetal bovine serum, Interleukin 1",
author = "Kandel, {Rita A.} and Pritzker, {Kenneth P H} and Gordon Mills and Cruz, {Tony F.}",
year = "1990",
month = "7",
day = "12",
doi = "10.1016/0167-4889(90)90004-W",
language = "English (US)",
volume = "1053",
pages = "130--134",
journal = "Biochimica et Biophysica Acta - Molecular Cell Research",
issn = "0167-4889",
publisher = "Elsevier",
number = "2-3",

}

TY - JOUR

T1 - Fetal bovine serum inhibits chondrocyte collagenase production

T2 - Interleukin 1 reverses this effect

AU - Kandel, Rita A.

AU - Pritzker, Kenneth P H

AU - Mills, Gordon

AU - Cruz, Tony F.

PY - 1990/7/12

Y1 - 1990/7/12

N2 - Bovine articular chondrocytes incubated in medium which was serum free or contained low levels of fetal bovine serum (less than 5%) constitutively produced collagenase. Increasing the concentration of serum in the culture medium inhibited the production of collagenase. Addition of interleukin 1 and lipopolysaccharide reversed the inhibitory effect of serum. Phorbol esters only stimulated collagenase production when the serum concentration was at least 10%. These data suggest that there is a factor(s) in fetal bovine serum that inhibits collagenase production by chondrocytes and this can be reversed by agents such as interleukin 1.

AB - Bovine articular chondrocytes incubated in medium which was serum free or contained low levels of fetal bovine serum (less than 5%) constitutively produced collagenase. Increasing the concentration of serum in the culture medium inhibited the production of collagenase. Addition of interleukin 1 and lipopolysaccharide reversed the inhibitory effect of serum. Phorbol esters only stimulated collagenase production when the serum concentration was at least 10%. These data suggest that there is a factor(s) in fetal bovine serum that inhibits collagenase production by chondrocytes and this can be reversed by agents such as interleukin 1.

KW - Chondrocyte

KW - Collagenase

KW - Fetal bovine serum

KW - Interleukin 1

UR - http://www.scopus.com/inward/record.url?scp=0025297290&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025297290&partnerID=8YFLogxK

U2 - 10.1016/0167-4889(90)90004-W

DO - 10.1016/0167-4889(90)90004-W

M3 - Article

VL - 1053

SP - 130

EP - 134

JO - Biochimica et Biophysica Acta - Molecular Cell Research

JF - Biochimica et Biophysica Acta - Molecular Cell Research

SN - 0167-4889

IS - 2-3

ER -