Feline immunodeficiency virus as a gene transfer vector in the rat nucleus tractus solitarii

L. H. Lin, J. E. Langasek, L. S. Talman, O. M. Taktakishvili, W. T. Talman

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Gene transfer has been used to examine the role of putative neurotransmitters in the nucleus tractus solitarii (NTS). Most such studies used adenovirus vector-mediated gene transfer although adenovirus vector transfects both neuronal and non-neuronal cells. Successful transfection in the NTS has also been reported with lentivirus as the vector. Feline immunodeficiency virus (FIV), a lentivirus, may preferentially transfect neurons and could be a powerful tool to delineate physiological effects produced by altered synthesis of transmitters in neurons. However, it has not been studied in NTS. Therefore, we sought to determine whether FIV transfects rat NTS cells and to define the type of cell transfected. We found that injection of FIV encoding LacZ gene (FIVLacZ) into the NTS led to transfection of numerous NTS cells. Injection of FIVLacZ did not alter immunoreactivity (IR) for neuronal nitric oxide synthase, which we have shown resides in NTS neurons. A majority (91.7 ± 3.9%) of transfected cells contained IR for neuronal nuclear antigen, a neuronal marker; 2.1 ± 3.8% of transfected cells contained IR for glial fibrillary acidic protein, a glial marker. No transfected neurons or fibers were observed in the nodose ganglion, which sends afferents to the NTS. We conclude that FIV almost exclusively transfects neurons in the rat NTS from which it is not retrogradely transported. The cell-type specificity of FIV in the NTS may provide a molecular method to study local physiological functions mediated by potential neurotransmitters in the NTS.

Original languageEnglish (US)
Pages (from-to)339-346
Number of pages8
JournalCellular and Molecular Neurobiology
Volume30
Issue number3
DOIs
StatePublished - Apr 2010
Externally publishedYes

Keywords

  • Feline immunodeficiency viral vector
  • Gene transfer
  • Neuronal marker
  • Nucleus tractus solitarii

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

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