Feeding induced by cannabinoids is mediated independently of the melanocortin system

Puspha Sinnayah, Erin E. Jobst, Joseph A. Rathner, Angela D. Caldera-Siu, Luciana Tonelli-Lemos, Aaron J. Eusterbrock, Pablo J. Enriori, Emmanuel N. Pothos, Kevin Grove, Michael A. Cowley

    Research output: Contribution to journalArticle

    26 Citations (Scopus)

    Abstract

    Background: Cannabinoids, the active components of marijuana, stimulate appetite, and cannabinoid receptor-1 (CB1-R) antagonists suppress appetite and promote weight loss. Little is known about how CB1-R antagonists affect the central neurocicuitry, specifically the melanocortin system that regulates energy balance. Methodology/Principal Finding: Here, we show that peripherally administered CB1-R antagonist (AM251) or agonist equally suppressed or stimulated feeding respectively in Ay, which lack a functional melanocortin system, and wildtype mice, demonstrating that cannabinoid effects on feeding do no require melanocortin circuitry. CB1-R antagonist or agonist administered into the ventral tegmental area (VTA) equally suppressed or stimulated feeding respectively, in both genotypes. In addition, peripheral and central cannabinoid administration similarly induced c-Fos activation in brain sites suggesting mediation via motivational dopaminergic circuitry. Amperometry-detected increases in evoked dopamine (DA) release by the CB1-R antagonist in nucleus accumbens slices indicates that AM251 modulates DA release from VTA terminals. Conclusions/Significance: Our results demonstrate that the effects of cannabinoids on energy balance are independent of hypothalamic melanocortin circuitry and is primarily driven by the reward system.

    Original languageEnglish (US)
    Article numbere2202
    JournalPLoS One
    Volume3
    Issue number5
    DOIs
    StatePublished - May 21 2008

    Fingerprint

    Melanocortins
    cannabinoids
    Cannabinoids
    antagonists
    Ventral Tegmental Area
    Appetite
    Energy balance
    Dopamine
    appetite
    dopamine
    energy balance
    agonists
    Cannabinoid Receptor CB1
    Cannabinoid Receptors
    Nucleus Accumbens
    Cannabis
    Cannabis sativa
    Reward
    Weight Loss
    Brain

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Medicine(all)

    Cite this

    Sinnayah, P., Jobst, E. E., Rathner, J. A., Caldera-Siu, A. D., Tonelli-Lemos, L., Eusterbrock, A. J., ... Cowley, M. A. (2008). Feeding induced by cannabinoids is mediated independently of the melanocortin system. PLoS One, 3(5), [e2202]. https://doi.org/10.1371/journal.pone.0002202

    Feeding induced by cannabinoids is mediated independently of the melanocortin system. / Sinnayah, Puspha; Jobst, Erin E.; Rathner, Joseph A.; Caldera-Siu, Angela D.; Tonelli-Lemos, Luciana; Eusterbrock, Aaron J.; Enriori, Pablo J.; Pothos, Emmanuel N.; Grove, Kevin; Cowley, Michael A.

    In: PLoS One, Vol. 3, No. 5, e2202, 21.05.2008.

    Research output: Contribution to journalArticle

    Sinnayah, P, Jobst, EE, Rathner, JA, Caldera-Siu, AD, Tonelli-Lemos, L, Eusterbrock, AJ, Enriori, PJ, Pothos, EN, Grove, K & Cowley, MA 2008, 'Feeding induced by cannabinoids is mediated independently of the melanocortin system', PLoS One, vol. 3, no. 5, e2202. https://doi.org/10.1371/journal.pone.0002202
    Sinnayah P, Jobst EE, Rathner JA, Caldera-Siu AD, Tonelli-Lemos L, Eusterbrock AJ et al. Feeding induced by cannabinoids is mediated independently of the melanocortin system. PLoS One. 2008 May 21;3(5). e2202. https://doi.org/10.1371/journal.pone.0002202
    Sinnayah, Puspha ; Jobst, Erin E. ; Rathner, Joseph A. ; Caldera-Siu, Angela D. ; Tonelli-Lemos, Luciana ; Eusterbrock, Aaron J. ; Enriori, Pablo J. ; Pothos, Emmanuel N. ; Grove, Kevin ; Cowley, Michael A. / Feeding induced by cannabinoids is mediated independently of the melanocortin system. In: PLoS One. 2008 ; Vol. 3, No. 5.
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