Feasibility of a polysaccharide isolated from Bletilla striata used as a gene vector administered through an interventional pathway

Xiang Wen Xia, Xin Li, Gan Sheng Feng, Chuan Sheng Zheng, Hui Min Liang, Xi Liu, Bin Xiong

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Abstract

AIM: To research into the feasibility of a polysaccharide isolated from Bletilla striata as a gene vector administered through an interventional pathway. METHODS: Bletilla striata polysaccharide (BSP) was isolated from a Chinese medicinal herb Bletilla striata, from which we prepared the cationic BSP (BSP+) by reductive amination. We further tested the ability of the BSP+ to incorporate and protect plasmid DNA, and the ability of the BSP+/pDNA complex to transfect into HepG2 cells. Then, we investigated the transfected effect of the BSP+/pDNA complex in vivo using green fluorescence protein (GFP) gene as a reporter gene administered through the hepatic artery. RESULTS: The synthesized cationic Bletilla striata polysaccharide (BSP+) incorporated and protected plasmid DNA to avoid enzymolysis by DNase. The transfection ratio was lower in the liposome group than in BSP+ group (28.87% ± 3.27% vs 36.64% ± 6.87%, P < 0.05). The BSP+/ pDNA complex was transfected into HepG2 cells in vitro and host cell in vivo. CONCLUSION: The BSP+/pDNA complex could target transfect into liver cell in vivo when administered through the hepatic artery using the interventional method, which could produce a marked effect as a new-type polycation gene vector in gene therapy.

Original languageEnglish (US)
Pages (from-to)1832-1835
Number of pages4
JournalWorld Chinese Journal of Digestology
Volume17
Issue number18
StatePublished - Jun 28 2009

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Keywords

  • Bletilla striata polysaccharide
  • Gene vector
  • Interventional
  • Reductive amination
  • Transfection

ASJC Scopus subject areas

  • Gastroenterology

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