Abstract
AIM: To research into the feasibility of a polysaccharide isolated from Bletilla striata as a gene vector administered through an interventional pathway. METHODS: Bletilla striata polysaccharide (BSP) was isolated from a Chinese medicinal herb Bletilla striata, from which we prepared the cationic BSP (BSP+) by reductive amination. We further tested the ability of the BSP+ to incorporate and protect plasmid DNA, and the ability of the BSP+/pDNA complex to transfect into HepG2 cells. Then, we investigated the transfected effect of the BSP+/pDNA complex in vivo using green fluorescence protein (GFP) gene as a reporter gene administered through the hepatic artery. RESULTS: The synthesized cationic Bletilla striata polysaccharide (BSP+) incorporated and protected plasmid DNA to avoid enzymolysis by DNase. The transfection ratio was lower in the liposome group than in BSP+ group (28.87% ± 3.27% vs 36.64% ± 6.87%, P <0.05). The BSP+/ pDNA complex was transfected into HepG2 cells in vitro and host cell in vivo. CONCLUSION: The BSP+/pDNA complex could target transfect into liver cell in vivo when administered through the hepatic artery using the interventional method, which could produce a marked effect as a new-type polycation gene vector in gene therapy.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1832-1835 |
| Number of pages | 4 |
| Journal | World Chinese Journal of Digestology |
| Volume | 17 |
| Issue number | 18 |
| State | Published - Jun 28 2009 |
| Externally published | Yes |
Fingerprint
Keywords
- Bletilla striata polysaccharide
- Gene vector
- Interventional
- Reductive amination
- Transfection
ASJC Scopus subject areas
- Gastroenterology
Cite this
Feasibility of a polysaccharide isolated from Bletilla striata used as a gene vector administered through an interventional pathway. / Xia, Xiang Wen; Li, Xin; Feng, Gan Sheng; Zheng, Chuan Sheng; Liang, Hui Min; Liu, Xi; Xiong, Bin.
In: World Chinese Journal of Digestology, Vol. 17, No. 18, 28.06.2009, p. 1832-1835.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Feasibility of a polysaccharide isolated from Bletilla striata used as a gene vector administered through an interventional pathway
AU - Xia, Xiang Wen
AU - Li, Xin
AU - Feng, Gan Sheng
AU - Zheng, Chuan Sheng
AU - Liang, Hui Min
AU - Liu, Xi
AU - Xiong, Bin
PY - 2009/6/28
Y1 - 2009/6/28
N2 - AIM: To research into the feasibility of a polysaccharide isolated from Bletilla striata as a gene vector administered through an interventional pathway. METHODS: Bletilla striata polysaccharide (BSP) was isolated from a Chinese medicinal herb Bletilla striata, from which we prepared the cationic BSP (BSP+) by reductive amination. We further tested the ability of the BSP+ to incorporate and protect plasmid DNA, and the ability of the BSP+/pDNA complex to transfect into HepG2 cells. Then, we investigated the transfected effect of the BSP+/pDNA complex in vivo using green fluorescence protein (GFP) gene as a reporter gene administered through the hepatic artery. RESULTS: The synthesized cationic Bletilla striata polysaccharide (BSP+) incorporated and protected plasmid DNA to avoid enzymolysis by DNase. The transfection ratio was lower in the liposome group than in BSP+ group (28.87% ± 3.27% vs 36.64% ± 6.87%, P <0.05). The BSP+/ pDNA complex was transfected into HepG2 cells in vitro and host cell in vivo. CONCLUSION: The BSP+/pDNA complex could target transfect into liver cell in vivo when administered through the hepatic artery using the interventional method, which could produce a marked effect as a new-type polycation gene vector in gene therapy.
AB - AIM: To research into the feasibility of a polysaccharide isolated from Bletilla striata as a gene vector administered through an interventional pathway. METHODS: Bletilla striata polysaccharide (BSP) was isolated from a Chinese medicinal herb Bletilla striata, from which we prepared the cationic BSP (BSP+) by reductive amination. We further tested the ability of the BSP+ to incorporate and protect plasmid DNA, and the ability of the BSP+/pDNA complex to transfect into HepG2 cells. Then, we investigated the transfected effect of the BSP+/pDNA complex in vivo using green fluorescence protein (GFP) gene as a reporter gene administered through the hepatic artery. RESULTS: The synthesized cationic Bletilla striata polysaccharide (BSP+) incorporated and protected plasmid DNA to avoid enzymolysis by DNase. The transfection ratio was lower in the liposome group than in BSP+ group (28.87% ± 3.27% vs 36.64% ± 6.87%, P <0.05). The BSP+/ pDNA complex was transfected into HepG2 cells in vitro and host cell in vivo. CONCLUSION: The BSP+/pDNA complex could target transfect into liver cell in vivo when administered through the hepatic artery using the interventional method, which could produce a marked effect as a new-type polycation gene vector in gene therapy.
KW - Bletilla striata polysaccharide
KW - Gene vector
KW - Interventional
KW - Reductive amination
KW - Transfection
UR - http://www.scopus.com/inward/record.url?scp=70349462634&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70349462634&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:70349462634
VL - 17
SP - 1832
EP - 1835
JO - World Chinese Journal of Digestology
JF - World Chinese Journal of Digestology
SN - 1009-3079
IS - 18
ER -