Abstract
AIM: To research into the feasibility of a polysaccharide isolated from Bletilla striata as a gene vector administered through an interventional pathway. METHODS: Bletilla striata polysaccharide (BSP) was isolated from a Chinese medicinal herb Bletilla striata, from which we prepared the cationic BSP (BSP+) by reductive amination. We further tested the ability of the BSP+ to incorporate and protect plasmid DNA, and the ability of the BSP+/pDNA complex to transfect into HepG2 cells. Then, we investigated the transfected effect of the BSP+/pDNA complex in vivo using green fluorescence protein (GFP) gene as a reporter gene administered through the hepatic artery. RESULTS: The synthesized cationic Bletilla striata polysaccharide (BSP+) incorporated and protected plasmid DNA to avoid enzymolysis by DNase. The transfection ratio was lower in the liposome group than in BSP+ group (28.87% ± 3.27% vs 36.64% ± 6.87%, P < 0.05). The BSP+/ pDNA complex was transfected into HepG2 cells in vitro and host cell in vivo. CONCLUSION: The BSP+/pDNA complex could target transfect into liver cell in vivo when administered through the hepatic artery using the interventional method, which could produce a marked effect as a new-type polycation gene vector in gene therapy.
Original language | English (US) |
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Pages (from-to) | 1832-1835 |
Number of pages | 4 |
Journal | World Chinese Journal of Digestology |
Volume | 17 |
Issue number | 18 |
DOIs | |
State | Published - Jun 28 2009 |
Keywords
- Bletilla striata polysaccharide
- Gene vector
- Interventional
- Reductive amination
- Transfection
ASJC Scopus subject areas
- Gastroenterology