Feasibility of a polysaccharide isolated from Bletilla striata used as a gene vector administered through an interventional pathway

Xiang Wen Xia, Xin Li, Gan Sheng Feng, Chuan Sheng Zheng, Hui Min Liang, Xi Liu, Bin Xiong

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

AIM: To research into the feasibility of a polysaccharide isolated from Bletilla striata as a gene vector administered through an interventional pathway. METHODS: Bletilla striata polysaccharide (BSP) was isolated from a Chinese medicinal herb Bletilla striata, from which we prepared the cationic BSP (BSP+) by reductive amination. We further tested the ability of the BSP+ to incorporate and protect plasmid DNA, and the ability of the BSP+/pDNA complex to transfect into HepG2 cells. Then, we investigated the transfected effect of the BSP+/pDNA complex in vivo using green fluorescence protein (GFP) gene as a reporter gene administered through the hepatic artery. RESULTS: The synthesized cationic Bletilla striata polysaccharide (BSP+) incorporated and protected plasmid DNA to avoid enzymolysis by DNase. The transfection ratio was lower in the liposome group than in BSP+ group (28.87% ± 3.27% vs 36.64% ± 6.87%, P <0.05). The BSP+/ pDNA complex was transfected into HepG2 cells in vitro and host cell in vivo. CONCLUSION: The BSP+/pDNA complex could target transfect into liver cell in vivo when administered through the hepatic artery using the interventional method, which could produce a marked effect as a new-type polycation gene vector in gene therapy.

Original languageEnglish (US)
Pages (from-to)1832-1835
Number of pages4
JournalWorld Chinese Journal of Digestology
Volume17
Issue number18
StatePublished - Jun 28 2009
Externally publishedYes

Fingerprint

Polysaccharides
Genes
Hepatic Artery
Hep G2 Cells
Plasmids
Amination
Deoxyribonucleases
DNA
Medicinal Plants
Reporter Genes
Liposomes
Genetic Therapy
Transfection
Fluorescence
Liver

Keywords

  • Bletilla striata polysaccharide
  • Gene vector
  • Interventional
  • Reductive amination
  • Transfection

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Feasibility of a polysaccharide isolated from Bletilla striata used as a gene vector administered through an interventional pathway. / Xia, Xiang Wen; Li, Xin; Feng, Gan Sheng; Zheng, Chuan Sheng; Liang, Hui Min; Liu, Xi; Xiong, Bin.

In: World Chinese Journal of Digestology, Vol. 17, No. 18, 28.06.2009, p. 1832-1835.

Research output: Contribution to journalArticle

Xia, Xiang Wen ; Li, Xin ; Feng, Gan Sheng ; Zheng, Chuan Sheng ; Liang, Hui Min ; Liu, Xi ; Xiong, Bin. / Feasibility of a polysaccharide isolated from Bletilla striata used as a gene vector administered through an interventional pathway. In: World Chinese Journal of Digestology. 2009 ; Vol. 17, No. 18. pp. 1832-1835.
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