Levels of c-myc mRNA have been assayed in mouse B cells cultured for 8 hr with Fab'2 anti-Ig or IgG anti-Ig. Fab'2 anti-Ig induces DNA synthesis in B cells, whereas the whole molecule inhibits anti-Ig-induced DNA synthesis by crosslinking the B cell Fcγ R to mIg. Both the Fab'2 fragment and the IgG anti-Ig induce an increase in c-myc mRNA by 1 hr. Thereafter, levels in cells stimulated with submitogenic doses of Fab'2 anti-Ig or any dose of IgG anti-Ig returned to background, while levels in cultures containing a mitogenic dose of Fab'2 anti-Ig remained elevated.
ASJC Scopus subject areas
- Molecular Biology