Fatty acid hydrolysis of acyl marinobactin siderophores by Marinobacter acylases

Michelle P. Kem, Hiroaki Naka, Akira Iinishi, Margo Haygood, Alison Butler

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The marine bacteria Marinobacter sp. DS40M6 and Marinobacter nanhaiticus D15-8W produce a suite of acyl peptidic marinobactin siderophores to acquire iron under iron-limiting conditions. During late-log phase growth, the marinobactins are hydrolyzed to form the marinobactin headgroup with release of the corresponding fatty acid tail. The bntA gene, a homologue of the Pseudomonas aeruginosa pyoverdine acylase gene, pvdQ, was identified from Marinobacter sp. DS40M6. A bntA knockout mutant of Marinobacter sp. DS40M6 produced the suite of acyl marinobactins A-E, without the usual formation of the marinobactin headgroup. Another marinobactin-producing species, M. nanhaiticus D15-8W, is predicted to have two pvdQ homologues, mhtA and mhtB. MhtA and MhtB have 67% identical amino acid sequences. MhtA catalyzes hydrolysis of the apo-marinobactin siderophores as well as the quorum sensing signaling molecule, dodecanoyl-homoserine lactone. In contrast to hydrolysis of the suite of apo-marinobactins by MhtA, hydrolysis of the iron(III)-bound marinobactins was not observed. (Chemical Equation Presented).

Original languageEnglish (US)
Pages (from-to)744-752
Number of pages9
JournalBiochemistry
Volume54
Issue number3
DOIs
StatePublished - Jan 27 2015

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amidase
Marinobacter
Siderophores
Hydrolysis
Fatty Acids
Iron
Genes
Quorum Sensing
Bacteria
Pseudomonas aeruginosa
Amino Acids
Amino Acid Sequence
Molecules
Growth

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)

Cite this

Kem, M. P., Naka, H., Iinishi, A., Haygood, M., & Butler, A. (2015). Fatty acid hydrolysis of acyl marinobactin siderophores by Marinobacter acylases. Biochemistry, 54(3), 744-752. https://doi.org/10.1021/bi5013673

Fatty acid hydrolysis of acyl marinobactin siderophores by Marinobacter acylases. / Kem, Michelle P.; Naka, Hiroaki; Iinishi, Akira; Haygood, Margo; Butler, Alison.

In: Biochemistry, Vol. 54, No. 3, 27.01.2015, p. 744-752.

Research output: Contribution to journalArticle

Kem, MP, Naka, H, Iinishi, A, Haygood, M & Butler, A 2015, 'Fatty acid hydrolysis of acyl marinobactin siderophores by Marinobacter acylases', Biochemistry, vol. 54, no. 3, pp. 744-752. https://doi.org/10.1021/bi5013673
Kem, Michelle P. ; Naka, Hiroaki ; Iinishi, Akira ; Haygood, Margo ; Butler, Alison. / Fatty acid hydrolysis of acyl marinobactin siderophores by Marinobacter acylases. In: Biochemistry. 2015 ; Vol. 54, No. 3. pp. 744-752.
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