Fasting activates neuropeptide Y neurons in the arcuate nucleus and the paraventricular nucleus in the rhesus macaque

Kevin Grove, Peilin Chen, Frank H. Koegler, Andrew Schiffmaker, M (Susan) Smith, Judy L. Cameron

    Research output: Contribution to journalArticle

    40 Citations (Scopus)

    Abstract

    It is well accepted that neuropeptide Y (NPY) plays a pivotal role in the regulation of food intake and energy homeostasis in the rodent, with NPY neurons in the arcuate nucleus (ARH) being thought of as the major contributor to the complex central feeding circuitry. Recent data from our group also indicate that NPY is important in the regulation of energy homeostasis in the nonhuman primate (NHP); exogenous NPY administration into the 3rd ventricle is a potent stimulator of food intake in the male rhesus macaque. The purpose of this study was to determine if NPY neurons in the rhesus macaque respond to a metabolic challenge, induced by 48 h of fasting, in a manner similar to that seen in the rodent. NPY mRNA was detected in hypothalamic sections from 48-h fasted or fed rhesus monkeys by in situ hybridization, using a [35S]UTP-labeled riboprobe specific for human NPY. Not surprisingly, NPY mRNA was abundant in the ARH of the NHP; however, of great interest was the expression of NPY mRNA in neurons within the paraventricular nucleus of the hypothalamus (PVH) and the supraoptic nucleus (SON). This raised the question as to whether all of these populations of NPY neurons are sensitive to changes in energy availability. Indeed, NPY expression in the ARH and PVH was significantly elevated in response to fasting; however, no significant change was detected in the SON. These data indicate that the NPY neurocircuitry involved in the regulation of food intake is more complex in the NHP than in rodents.

    Original languageEnglish (US)
    Pages (from-to)133-138
    Number of pages6
    JournalMolecular Brain Research
    Volume113
    Issue number1-2
    DOIs
    StatePublished - May 12 2003

    Fingerprint

    Arcuate Nucleus of Hypothalamus
    Paraventricular Hypothalamic Nucleus
    Neuropeptide Y
    Macaca mulatta
    Fasting
    Neurons
    Primates
    Appetite Regulation
    Rodentia
    Supraoptic Nucleus
    Messenger RNA
    Homeostasis
    Uridine Triphosphate
    Third Ventricle
    Hypothalamus
    In Situ Hybridization
    Eating

    Keywords

    • ARH
    • Feeding
    • Hypothalamus
    • Nonhuman primate
    • NPY
    • PVH

    ASJC Scopus subject areas

    • Molecular Biology
    • Cellular and Molecular Neuroscience

    Cite this

    Fasting activates neuropeptide Y neurons in the arcuate nucleus and the paraventricular nucleus in the rhesus macaque. / Grove, Kevin; Chen, Peilin; Koegler, Frank H.; Schiffmaker, Andrew; Smith, M (Susan); Cameron, Judy L.

    In: Molecular Brain Research, Vol. 113, No. 1-2, 12.05.2003, p. 133-138.

    Research output: Contribution to journalArticle

    Grove, Kevin ; Chen, Peilin ; Koegler, Frank H. ; Schiffmaker, Andrew ; Smith, M (Susan) ; Cameron, Judy L. / Fasting activates neuropeptide Y neurons in the arcuate nucleus and the paraventricular nucleus in the rhesus macaque. In: Molecular Brain Research. 2003 ; Vol. 113, No. 1-2. pp. 133-138.
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