Fast and multiplexed superresolution imaging with DNA-PAINT-ERS

Fehmi Civitci; Julia Shangguan; Ting Zheng; Kai Tao; Matthew Rames; John Kenison; Ying Zhang; Lei Wu; Carey Phelps; Sadik Esener; Xiaolin Nan

doi:10.1038/s41467-020-18181-6

Fast and multiplexed superresolution imaging with DNA-PAINT-ERS

Fehmi Civitci, Julia Shangguan, Ting Zheng, Kai Tao, Matthew Rames, John Kenison, Ying Zhang, Lei Wu, Carey Phelps, Sadik Esener, Xiaolin Nan

Research output: Contribution to journal › Article

Abstract

DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) facilitates multiplexing in superresolution microscopy but is practically limited by slow imaging speed. To address this issue, we propose the additions of ethylene carbonate (EC) to the imaging buffer, sequence repeats to the docking strand, and a spacer between the docking strand and the affinity agent. Collectively termed DNA-PAINT-ERS (E = EC, R = Repeating sequence, and S = Spacer), these strategies can be easily integrated into current DNA-PAINT workflows for both accelerated imaging speed and improved image quality through optimized DNA hybridization kinetics and efficiency. We demonstrate the general applicability of DNA-PAINT-ERS for fast, multiplexed superresolution imaging using previously validated oligonucleotide constructs with slight modifications.

Original language	English (US)
Article number	4339
Journal	Nature communications
Volume	11
Issue number	1
DOIs	https://doi.org/10.1038/s41467-020-18181-6
State	Published - Dec 1 2020

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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abstract = "DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) facilitates multiplexing in superresolution microscopy but is practically limited by slow imaging speed. To address this issue, we propose the additions of ethylene carbonate (EC) to the imaging buffer, sequence repeats to the docking strand, and a spacer between the docking strand and the affinity agent. Collectively termed DNA-PAINT-ERS (E = EC, R = Repeating sequence, and S = Spacer), these strategies can be easily integrated into current DNA-PAINT workflows for both accelerated imaging speed and improved image quality through optimized DNA hybridization kinetics and efficiency. We demonstrate the general applicability of DNA-PAINT-ERS for fast, multiplexed superresolution imaging using previously validated oligonucleotide constructs with slight modifications.",

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AU - Civitci, Fehmi

AU - Shangguan, Julia

AU - Zheng, Ting

AU - Tao, Kai

AU - Rames, Matthew

AU - Kenison, John

AU - Zhang, Ying

AU - Wu, Lei

AU - Phelps, Carey

AU - Esener, Sadik

AU - Nan, Xiaolin

PY - 2020/12/1

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