TY - JOUR
T1 - Fast and multiplexed superresolution imaging with DNA-PAINT-ERS
AU - Civitci, Fehmi
AU - Shangguan, Julia
AU - Zheng, Ting
AU - Tao, Kai
AU - Rames, Matthew
AU - Kenison, John
AU - Zhang, Ying
AU - Wu, Lei
AU - Phelps, Carey
AU - Esener, Sadik
AU - Nan, Xiaolin
N1 - Funding Information:
The authors thank Drs. Joe W Gray, Bruce Branchaud, Paul Spellman, Sunjong Kwon, Daniel Heineck, Yu-Jui (Roger) Chiu, and other colleagues at OHSU for their helpful discussions. Research in the Nan lab was supported by the OHSU Knight Cancer Institute, the Damon Runyon Cancer Research Foundation, the M. J. Murdock Charitable Trust, the Prospect Creek Foundation, the Cancer Systems Biology Consortium from the National Cancer Institute (CSBC, grant number U54 CA209988, PI: Joe W. Gray), and the National Institute of General Medical Sciences (grant number R01 GM132322, PI: X.N.). F.C., T.Z., M.R., J.K., S.E., and X.N. are members of and supported by the Caner Early Detection Advanced Research (CEDAR) Center of the OHSU Knight Cancer Institute.
Publisher Copyright:
© 2020, The Author(s).
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/12/1
Y1 - 2020/12/1
N2 - DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) facilitates multiplexing in superresolution microscopy but is practically limited by slow imaging speed. To address this issue, we propose the additions of ethylene carbonate (EC) to the imaging buffer, sequence repeats to the docking strand, and a spacer between the docking strand and the affinity agent. Collectively termed DNA-PAINT-ERS (E = EC, R = Repeating sequence, and S = Spacer), these strategies can be easily integrated into current DNA-PAINT workflows for both accelerated imaging speed and improved image quality through optimized DNA hybridization kinetics and efficiency. We demonstrate the general applicability of DNA-PAINT-ERS for fast, multiplexed superresolution imaging using previously validated oligonucleotide constructs with slight modifications.
AB - DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) facilitates multiplexing in superresolution microscopy but is practically limited by slow imaging speed. To address this issue, we propose the additions of ethylene carbonate (EC) to the imaging buffer, sequence repeats to the docking strand, and a spacer between the docking strand and the affinity agent. Collectively termed DNA-PAINT-ERS (E = EC, R = Repeating sequence, and S = Spacer), these strategies can be easily integrated into current DNA-PAINT workflows for both accelerated imaging speed and improved image quality through optimized DNA hybridization kinetics and efficiency. We demonstrate the general applicability of DNA-PAINT-ERS for fast, multiplexed superresolution imaging using previously validated oligonucleotide constructs with slight modifications.
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U2 - 10.1038/s41467-020-18181-6
DO - 10.1038/s41467-020-18181-6
M3 - Article
C2 - 32859909
AN - SCOPUS:85089973043
VL - 11
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
IS - 1
M1 - 4339
ER -