Fast adaptation in vestibular hair cells depends on MYOSIN-1C

P. G. Gillespie, J. D. Scarborough, J. A. Mercer, E. Stauffer, J. R. Holt

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Fast adaptation, the rapid termination of transduction current that follows a mechanical stimulus, correlates temporally with hair-bundle force production that could boost bundle displacement in response to weak stimuli. We have presented data in favor of the "release model", which proposes that Ca2+ lengthens a mechanical linkage in the transduction apparatus, reducing gating-spring tension and allowing channels to close. To determine the molecule responsible for fast adaptation, we introduced the Y61G mutation into the Myo1c genomic locus using gene targeting. We show here that both fast and slow slipping adaptation are faster in Y61G knock-in hair cells as compared to C57BL/6 control cells. Although these results show that mechanical activity of myosin-1c is required for fast adaptation in vestibular hair cells, additional controls are required to ensure that the differences in adaptation rate are not due to strain differences between the two mouse lines.

Original languageEnglish (US)
Title of host publicationAuditory Mechanisms
Subtitle of host publicationProcesses and Models - Proceedings of the 9th International Symposium
EditorsAlfred L. Nuttall, Tianying Ren, Peter Gillespie, Karl Grosh, Egbert de Boer
PublisherWorld Scientific Publishing Co. Pte Ltd
Pages169-175
Number of pages7
ISBN (Electronic)9812568247, 9789812568243
StatePublished - 2005
Event9th International Mechanics of Hearing Workshop on Auditory Mechanisms: Processes and Models, MoH 2005 - Portland, United States
Duration: Jul 23 2005Jul 28 2005

Publication series

NameAuditory Mechanisms: Processes and Models - Proceedings of the 9th International Symposium

Conference

Conference9th International Mechanics of Hearing Workshop on Auditory Mechanisms: Processes and Models, MoH 2005
CountryUnited States
CityPortland
Period7/23/057/28/05

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering

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  • Cite this

    Gillespie, P. G., Scarborough, J. D., Mercer, J. A., Stauffer, E., & Holt, J. R. (2005). Fast adaptation in vestibular hair cells depends on MYOSIN-1C. In A. L. Nuttall, T. Ren, P. Gillespie, K. Grosh, & E. de Boer (Eds.), Auditory Mechanisms: Processes and Models - Proceedings of the 9th International Symposium (pp. 169-175). (Auditory Mechanisms: Processes and Models - Proceedings of the 9th International Symposium). World Scientific Publishing Co. Pte Ltd.