FAK promotes organization of fibronectin matrix and fibrillar adhesions

Duško Ilić, Branka Kovačič, Kohei Johkura, David D. Schlaepfer, Nenad Tomašević, Qin Han, Jae Beom Kim, Kyle Howerton, Clark Baumbusch, Naoko Ogiwara, Daniel Streblow, Jay Nelson, Paul Dazin, Yuji Shino, Katsunori Sasaki, Caroline H. Damsky

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Targeted disruption of the focal adhesion kinase (FAK) gene in mice is lethal at embryonic day 8.5 (E8.5). Vascular defects in FAK-/- mice result from the inability of FAK-deficient endothelial cells to organize themselves into vascular network. We found that, although fibronectin (FN) levels were similar, its organization was less fibrillar in both FAK-/- endothelial cells and mesoderm of E8.5 FAK-/- embryos, as well as in mouse embryonic fibroblasts isolated from mutant embryos. FAK catalytic activity, proline-rich domains, and location in focal contacts were all required for proper allocation and patterning of FN matrix. Cells lacking FAK in focal adhesions fail to translocate supramolecular complexes of integrin-bound FN and focal adhesion proteins along actin filaments to form mature fibrillar adhesions. Taken together, our data suggest that proper FN allocation and organization are dependent on FAK-mediated remodeling of focal adhesions.

Original languageEnglish (US)
Pages (from-to)177-187
Number of pages11
JournalJournal of Cell Science
Volume117
Issue number2
DOIs
StatePublished - Jan 15 2004

Fingerprint

Focal Adhesion Protein-Tyrosine Kinases
Fibronectins
Focal Adhesions
Blood Vessels
Embryonic Structures
Endothelial Cells
Mesoderm
Actin Cytoskeleton
Proline
Integrins
Fibroblasts
Genes

Keywords

  • FAK
  • Fibrillar adhesions
  • Fibronectin matrix allocation
  • Focal adhesion dynamics
  • Motility

ASJC Scopus subject areas

  • Cell Biology

Cite this

Ilić, D., Kovačič, B., Johkura, K., Schlaepfer, D. D., Tomašević, N., Han, Q., ... Damsky, C. H. (2004). FAK promotes organization of fibronectin matrix and fibrillar adhesions. Journal of Cell Science, 117(2), 177-187. https://doi.org/10.1242/jcs.00845

FAK promotes organization of fibronectin matrix and fibrillar adhesions. / Ilić, Duško; Kovačič, Branka; Johkura, Kohei; Schlaepfer, David D.; Tomašević, Nenad; Han, Qin; Kim, Jae Beom; Howerton, Kyle; Baumbusch, Clark; Ogiwara, Naoko; Streblow, Daniel; Nelson, Jay; Dazin, Paul; Shino, Yuji; Sasaki, Katsunori; Damsky, Caroline H.

In: Journal of Cell Science, Vol. 117, No. 2, 15.01.2004, p. 177-187.

Research output: Contribution to journalArticle

Ilić, D, Kovačič, B, Johkura, K, Schlaepfer, DD, Tomašević, N, Han, Q, Kim, JB, Howerton, K, Baumbusch, C, Ogiwara, N, Streblow, D, Nelson, J, Dazin, P, Shino, Y, Sasaki, K & Damsky, CH 2004, 'FAK promotes organization of fibronectin matrix and fibrillar adhesions', Journal of Cell Science, vol. 117, no. 2, pp. 177-187. https://doi.org/10.1242/jcs.00845
Ilić D, Kovačič B, Johkura K, Schlaepfer DD, Tomašević N, Han Q et al. FAK promotes organization of fibronectin matrix and fibrillar adhesions. Journal of Cell Science. 2004 Jan 15;117(2):177-187. https://doi.org/10.1242/jcs.00845
Ilić, Duško ; Kovačič, Branka ; Johkura, Kohei ; Schlaepfer, David D. ; Tomašević, Nenad ; Han, Qin ; Kim, Jae Beom ; Howerton, Kyle ; Baumbusch, Clark ; Ogiwara, Naoko ; Streblow, Daniel ; Nelson, Jay ; Dazin, Paul ; Shino, Yuji ; Sasaki, Katsunori ; Damsky, Caroline H. / FAK promotes organization of fibronectin matrix and fibrillar adhesions. In: Journal of Cell Science. 2004 ; Vol. 117, No. 2. pp. 177-187.
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