Fah knockout animals as models for therapeutic liver repopulation

Research output: Chapter in Book/Report/Conference proceedingChapter

11 Scopus citations

Abstract

Several animal models of Fah deficiency have been developed, including mice, pigs and most recently rats. Initially, the murine models were developed with the intent to mirror the human disease for pathophysiologic and therapeutic studies. However, it soon became apparent that Fah-positive hepatocytes have a potent selective growth advantage in mutant liver and can extensively repopulate the diseased organ. For this reason, Fah mutant mice have become a workhorse for liver biology and are widely used in liver stem cell and hepatic gene therapy research. Immune deficient Fah-knockout mice can be repopulated with human hepatocytes, creating “mice with human livers”. These chimeric animals have become an important preclinical model for infectious diseases, metabolism and gene therapy. The potent expansion of human hepatocytes in Fah knockout mice has given rise to the concept of using Fah mutants as living bioreactors to produce large quantities of fully mature hepatocytes. As a consequence, larger animal models of Fah deficiency have recently been developed.

Original languageEnglish (US)
Title of host publicationAdvances in Experimental Medicine and Biology
PublisherSpringer New York LLC
Pages215-230
Number of pages16
Volume959
DOIs
StatePublished - 2017

Publication series

NameAdvances in Experimental Medicine and Biology
Volume959
ISSN (Print)0065-2598
ISSN (Electronic)2214-8019

Keywords

  • Animal model
  • Cell transplantation
  • Chimeric animals
  • Gene therapy
  • Hepatocellular carcinoma
  • Hepatocyte
  • Liver repopulation
  • Selective advantage

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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