Factor xliia binds microvascular endothelial cells and modulates angiogenesis in vitro

L. A. Falls, S. M. Dallabrida, David Farrell

Research output: Contribution to journalArticle

Abstract

Factor Xlll-deficient patients have impaired wound healing, but the mechanistic role of factor XIII in wound healing remains unclear. Our work shows the human microvascular endothelial cell line, HMEC-1, binds immobilized factor XIIla, but not iodoacetamide-inactivated factor XHIa, in a dose-dependent manner. Binding of soluble factor Xllla to HMEC-ls was confirmed by fluorescence activated cell sorting analysis. An antibody to β1 integrins blocked binding of HMEC-ls to factor Xllla 60%, while an antibody to avβz decreased binding 70%. and a combination of the antibodies reduced binding >95%. To examine the role of factor Xllla in wound repair, a three-dimensional microcarrier-based in vitro angiogenesis assay was conducted. Factor XIII was removed from γ A/γA and γA/γfibrinogen by urea treatment. HMEC- Is stimulated for angiogenesis with basic fibroblast growth factor or vascular endothelial growth factor in γA/-γA and γA/-γ fibrin clots showed decreased cell migration and sprout length in the presence of factor Xllla, however, the number of capillary-like sprouts per microcarrier remained unchanged. In conclusion, factor Xllla may play a role in wound repair by binding microvascular endothelial cells in an integrin dependent manner and affecting cell migration and sprout length during angiogenesis.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number8
StatePublished - 1998
Externally publishedYes

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Endothelial cells
angiogenesis
endothelial cells
Factor XIII
Endothelial Cells
integrins
tissue repair
cell movement
Integrins
animal injuries
Wound Healing
antibodies
Cell Movement
Antibodies
Repair
vascular endothelial growth factor A
Iodoacetamide
fibroblast growth factor 2
fibrin
Wounds and Injuries

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Factor xliia binds microvascular endothelial cells and modulates angiogenesis in vitro. / Falls, L. A.; Dallabrida, S. M.; Farrell, David.

In: FASEB Journal, Vol. 12, No. 8, 1998.

Research output: Contribution to journalArticle

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