Facile synthesis of symmetric, monofunctional cyanine dyes for imaging applications

Lai Qiang Ying, Bruce P. Branchaud

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Efficient syntheses of several members of a new class of symmetric, monocarboxylate-functionalized cyanine dyes have been developed. The synthesis is a simple two-step method, typically with greater than 60% yield and easy final product purification. The new monocarboxylate-functionalized cyanine dyes exhibit excellent water solubility and similar excitation and emission properties to those of Cy5 and Alexa Fluor 647. The application of the new dyes in cellular imaging has been demonstrated through direct conjugating of the dye with an antibody, then imaging of microtubules inside cells, visualized by near-infrared fluorescence microscopy.

Original languageEnglish (US)
Pages (from-to)865-869
Number of pages5
JournalBioconjugate Chemistry
Volume22
Issue number4
DOIs
StatePublished - Apr 20 2011

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

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