TY - JOUR
T1 - F-actin levels but not actin polymerization are affected by triphenyltin in HL-60 cells
AU - Marinovich, Marina
AU - Guizzetti, Marina
AU - Grazi, Enrico
AU - Trombetta, Giorgio
AU - Galli, Corrado Lodovico
N1 - Funding Information:
This study was partially supported by an EU grant EVjV-CT94-0508 (DG 12 SOLS) and by a 407~ project of MURST. We thank Dr. P. Woodford for kindly revising the manuscript.
PY - 1996/2/15
Y1 - 1996/2/15
N2 - The toxin triphenyl tin (TPT), Sn(C6H5)3+ caused a rapid decrease in the F-actin content of promyelocytic human leukemia cells (HL-60) chemically differentiated to neutrophils. Prior incubation (2 min) of the cells with 10 μM TPT did not modify the extent of actin polymerization inducible either by a receptor-mediated stimulus (chemotactic peptide fMLP) or by a direct activator of G proteins (AIF4-). The inorganic tin salts SnCl4, and SnCl4 did not affect F-actin content or production of HL-60 cells. Microfilament thiol groups were not reduced by exposure of cells to TPT, but even increased. When F-actin was exposed to 10 μM triphenyltin in a cell-free system, the depolymerizing effect was not detectable. Thus, TPT does not affect cytoskeletal protein directly but depends for its toxicity on some other induced change, probably ionic/osmotic in the intact cell.
AB - The toxin triphenyl tin (TPT), Sn(C6H5)3+ caused a rapid decrease in the F-actin content of promyelocytic human leukemia cells (HL-60) chemically differentiated to neutrophils. Prior incubation (2 min) of the cells with 10 μM TPT did not modify the extent of actin polymerization inducible either by a receptor-mediated stimulus (chemotactic peptide fMLP) or by a direct activator of G proteins (AIF4-). The inorganic tin salts SnCl4, and SnCl4 did not affect F-actin content or production of HL-60 cells. Microfilament thiol groups were not reduced by exposure of cells to TPT, but even increased. When F-actin was exposed to 10 μM triphenyltin in a cell-free system, the depolymerizing effect was not detectable. Thus, TPT does not affect cytoskeletal protein directly but depends for its toxicity on some other induced change, probably ionic/osmotic in the intact cell.
KW - F-actin
KW - HL-60 cell
KW - Triphenyltin
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U2 - 10.1016/1382-6689(95)00004-6
DO - 10.1016/1382-6689(95)00004-6
M3 - Article
C2 - 21781658
AN - SCOPUS:0030001920
SN - 1382-6689
VL - 1
SP - 13
EP - 20
JO - Environmental Toxicology and Pharmacology
JF - Environmental Toxicology and Pharmacology
IS - 1
ER -