Extrasynaptic NMDA receptors on rod pathway amacrine cells: Molecular composition, activation, and signaling

Margaret L. Veruki, Yifan Zhou, Áurea Castilho, Catherine Morgans, Espen Hartveit

    Research output: Contribution to journalArticle

    Abstract

    In the rod pathway of the mammalian retina, axon terminals of glutamatergic rod bipolar cells are presynaptic to AII and A17 amacrine cells in the inner plexiform layer. Recent evidence suggests that both amacrines express NMDA receptors, raising questions concerning molecular composition, localization, activation, and function of these receptors. Using dual patch-clamp recording from synaptically connected rod bipolar and AII or A17 amacrine cells in retinal slices from female rats, we found no evidence that NMDA receptors contribute to postsynaptic currents evoked in either amacrine. Instead, NMDA receptors on both amacrine cells were activated by ambient glutamate, and blocking glutamate uptake increased their level of activation. NMDA receptor activation also increased the frequency of GABAergic postsynaptic currents in rod bipolar cells, suggesting that NMDA receptors can drive release of GABA from A17 amacrines. A striking dichotomy was revealed by pharmacological and immunolabeling experiments, which found GluN2B-containing NMDA receptors on AII amacrines and GluN2A-containing NMDA receptors on A17 amacrines. Immunolabeling also revealed a clustered organization of NMDA receptors on both amacrines and a close spatial association between GluN2B subunits and connexin 36 on AII amacrines, suggesting that NMDA receptor modulation of gap junction coupling between these cells involves the GluN2B subunit. Using multiphoton Ca2+ imaging, we verified that activation of NMDA receptors evoked an increase of intracellular Ca2+ in dendrites of both amacrines. Our results suggest that AII and A17 amacrines express clustered, extrasynaptic NMDA receptors, with different and complementary subunits that are likely to contribute differentially to signal processing and plasticity.

    Original languageEnglish (US)
    Pages (from-to)627-650
    Number of pages24
    JournalJournal of Neuroscience
    Volume39
    Issue number4
    DOIs
    StatePublished - Jan 23 2019

    Fingerprint

    Amacrine Cells
    N-Methyl-D-Aspartate Receptors
    A 17
    Synaptic Potentials
    Glutamic Acid
    Gap Junctions
    Presynaptic Terminals
    Dendrites
    gamma-Aminobutyric Acid
    Retina

    Keywords

    • Amacrine cells
    • GluN2A
    • GluN2B
    • NMDA receptors
    • Patch-clamp
    • Retina

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Extrasynaptic NMDA receptors on rod pathway amacrine cells : Molecular composition, activation, and signaling. / Veruki, Margaret L.; Zhou, Yifan; Castilho, Áurea; Morgans, Catherine; Hartveit, Espen.

    In: Journal of Neuroscience, Vol. 39, No. 4, 23.01.2019, p. 627-650.

    Research output: Contribution to journalArticle

    Veruki, Margaret L. ; Zhou, Yifan ; Castilho, Áurea ; Morgans, Catherine ; Hartveit, Espen. / Extrasynaptic NMDA receptors on rod pathway amacrine cells : Molecular composition, activation, and signaling. In: Journal of Neuroscience. 2019 ; Vol. 39, No. 4. pp. 627-650.
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