Extracellular matrix production and regulation in micropatterned endothelial cells

Deirdre E J Anderson, Monica Hinds

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Production and maintenance of extracellular matrix (ECM) is an essential aspect of endothelial cell (EC) function. ECM surfaces composed of collagen type IV and laminin support an atheroprotective endothelium, while fibronectin may encourage an atheroprone endothelium through inflammation or wound repair signaling. ECs maintain this underlying structure through regulation of protein production and degradation, yet the role of cytoskeletal alignment on this regulation is unknown. To examine the regulation and production of ECM by ECs with an atheroprotective phenotype, ECs were micropatterned onto lanes, which created an elongated EC morphology similar to that seen with unidirectional fluid shear stress application. Collagen IV and fibronectin protein production were measured as were gene expression of collagen IV, fibronectin, laminin, MMP2, MMP9, TIMP1, TIMP2, and TGF-β1. ECs were also treated with TNF to simulate an injury model. Micropattern-induced elongation led to significant increases in collagen IV and fibronectin protein production, and collagen IV, laminin, and TGF-β1 gene expression, but no significant changes in the MMP or TIMP genes. TNF treatment significantly increased collagen IV gene and protein production. These results suggest that the increase in ECM synthesis in micropattern-elongated ECs is likely regulated with TGF-β1, and this increase in ECM could be relevant to the atheroprotection needed for maintenance of a healthy endothelium in vivo.

Original languageEnglish (US)
Pages (from-to)159-164
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume427
Issue number1
DOIs
StatePublished - Oct 12 2012

Fingerprint

Endothelial cells
Extracellular Matrix
Collagen
Fibronectins
Endothelial Cells
Endothelium
Laminin
Gene expression
Maintenance
Proteins
Gene Expression
Collagen Type IV
Wounds and Injuries
Matrix Metalloproteinases
Proteolysis
Shear stress
Elongation
Inflammation
Repair
Phenotype

Keywords

  • Collagen
  • Endothelial cells
  • Fibronectin
  • Micropattern
  • TGF-β
  • TNF

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Extracellular matrix production and regulation in micropatterned endothelial cells. / Anderson, Deirdre E J; Hinds, Monica.

In: Biochemical and Biophysical Research Communications, Vol. 427, No. 1, 12.10.2012, p. 159-164.

Research output: Contribution to journalArticle

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