Expression profiling of herpesvirus and vaccinia virus proteins using a high-throughput baculovirus screening system

Mian Gao; Nicole Brufatto; Tricia Chen; Laura Lea Murley; Rosanne Thalakada; Megan Domagala; Bryan Beattie; Daniel Mamelak; Vicki Athanasopoulos; David Johnson; Grant McFadden; Christian Burks; Lori Frappier

doi:10.1021/pr050137u

Expression profiling of herpesvirus and vaccinia virus proteins using a high-throughput baculovirus screening system

Mian Gao, Nicole Brufatto, Tricia Chen, Laura Lea Murley, Rosanne Thalakada, Megan Domagala, Bryan Beattie, Daniel Mamelak, Vicki Athanasopoulos, David Johnson, Grant McFadden, Christian Burks, Lori Frappier

Molecular Microbiology and Immunology

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

We have developed a high-throughput system for generating baculoviruses and testing the expression, solubility, and affinity column purification of encoded proteins. We have used this system to generate baculoviruses for and analyze the expression of 337 proteins from three different herpesviruses (HSV-1, EBV, and CMV) and vaccinia virus. Subsets of these proteins were also tested for expression and solubility in E. coli. Comparisons of the results in the two systems are presented for each virus.

Original language	English (US)
Pages (from-to)	2225-2235
Number of pages	11
Journal	Journal of Proteome Research
Volume	4
Issue number	6
DOIs	https://doi.org/10.1021/pr050137u
State	Published - Nov 2005

Keywords

Baculovirus
Cytomegalovirus
Epstein-Barr virus
Herpes simplex virus
High-throughput protein expression
Vaccinia

ASJC Scopus subject areas

General Chemistry
Biochemistry

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10.1021/pr050137u

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Gao, M., Brufatto, N., Chen, T., Murley, L. L., Thalakada, R., Domagala, M., Beattie, B., Mamelak, D., Athanasopoulos, V., Johnson, D., McFadden, G., Burks, C., & Frappier, L. (2005). Expression profiling of herpesvirus and vaccinia virus proteins using a high-throughput baculovirus screening system. Journal of Proteome Research, 4(6), 2225-2235. https://doi.org/10.1021/pr050137u

Gao, M, Brufatto, N, Chen, T, Murley, LL, Thalakada, R, Domagala, M, Beattie, B, Mamelak, D, Athanasopoulos, V, Johnson, D, McFadden, G, Burks, C & Frappier, L 2005, 'Expression profiling of herpesvirus and vaccinia virus proteins using a high-throughput baculovirus screening system', Journal of Proteome Research, vol. 4, no. 6, pp. 2225-2235. https://doi.org/10.1021/pr050137u

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keywords = "Baculovirus, Cytomegalovirus, Epstein-Barr virus, Herpes simplex virus, High-throughput protein expression, Vaccinia",

author = "Mian Gao and Nicole Brufatto and Tricia Chen and Murley, {Laura Lea} and Rosanne Thalakada and Megan Domagala and Bryan Beattie and Daniel Mamelak and Vicki Athanasopoulos and David Johnson and Grant McFadden and Christian Burks and Lori Frappier",

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T1 - Expression profiling of herpesvirus and vaccinia virus proteins using a high-throughput baculovirus screening system

AU - Gao, Mian

AU - Brufatto, Nicole

AU - Chen, Tricia

AU - Murley, Laura Lea

AU - Thalakada, Rosanne

AU - Domagala, Megan

AU - Beattie, Bryan

AU - Mamelak, Daniel

AU - Athanasopoulos, Vicki

AU - Johnson, David

AU - McFadden, Grant

AU - Burks, Christian

AU - Frappier, Lori

PY - 2005/11

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AB - We have developed a high-throughput system for generating baculoviruses and testing the expression, solubility, and affinity column purification of encoded proteins. We have used this system to generate baculoviruses for and analyze the expression of 337 proteins from three different herpesviruses (HSV-1, EBV, and CMV) and vaccinia virus. Subsets of these proteins were also tested for expression and solubility in E. coli. Comparisons of the results in the two systems are presented for each virus.

KW - Baculovirus

KW - Cytomegalovirus

KW - Epstein-Barr virus

KW - Herpes simplex virus

KW - High-throughput protein expression

KW - Vaccinia

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Expression profiling of herpesvirus and vaccinia virus proteins using a high-throughput baculovirus screening system

Abstract

Keywords

ASJC Scopus subject areas

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