Expression profiling of genes encoding glutamate and GABA receptor subunits in three immortalized GnRH cell lines

Vasilios T. Garyfallou, Dario Lemos, Henryk F. Urbanski

    Research output: Contribution to journalArticlepeer-review

    11 Scopus citations

    Abstract

    Gonadotropin-releasing hormone (GnRH) plays a central role in regulating development and function of the reproductive axis, and its secretion is known to be influenced by glutamate and GABA. In the present study, we used gene microarrays and RT-PCR to compare the expression profiles of glutamate and GABA receptor subunits in three immortalized GnRH cell lines: GT1-1, GT1-7, and Gn10. All of these cell lines expressed the AMPA glutamate receptor subunit genes GluR2 and GluR4, but only the GT1-1 and GT1-7 cells expressed the kainate glutamate receptor subunit gene KA2. Additionally, GluRδ2, a subunit that can form heteromeric receptors with kainate and AMPA subunits, was present in GT1-1 and Gn10 cells but not in GT1-7 cells. Genes encoding the GABAA receptor α3, β2, β3, ε, and π subunits, as well as the GABAB receptor 1 subunit, were evident in all three cell lines. However, the gene encoding the expression of GABAA receptor γ subunit was noticeably absent. Taken together, these data demonstrate comprehensive screening of neurotransmitter receptor genes in a controlled neuronal culture system, and reveal novel features.

    Original languageEnglish (US)
    Pages (from-to)50-54
    Number of pages5
    JournalBrain research
    Volume1086
    Issue number1
    DOIs
    StatePublished - May 1 2006

    Keywords

    • GT1-1 cells
    • GT1-7 cells
    • Gene microarray
    • Gn10 cells
    • Gonadotropin-releasing hormone
    • Reproduction

    ASJC Scopus subject areas

    • Neuroscience(all)
    • Molecular Biology
    • Clinical Neurology
    • Developmental Biology

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