Expression profiling identifies strain-specific changes associated with ethanol withdrawal in mice

G. M. Daniels, Kari Buck

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Mice that exhibit characteristics of physical dependence following ethanol exposure serve as useful models of alcoholism in humans. The DBA/2J and C57BL/ 6J inbred strains differ in their behavioral response to ethanol withdrawal. Alterations in gene expression are believed to underlie neuroadaptation to ethanol dependence and tolerance. Therefore, the differences in ethanol withdrawal severity observed between the DBA/2J and C57BL/6J strains may be related to differential regulation of gene expression. We have used cDNA microarrays to determine the gene expression profile in the hippocampus of DBA/2J and C57BL/6J mice during withdrawal after chronic and acute ethanol exposure. Of the 7634 genes surveyed, approximately 2% were consistently differentially expressed by at least 1.4-fold in DBA/2J mice during chronic ethanol withdrawal. Less than 1% of the genes showed altered expression in C57BL/6J mice under the same conditions, or in DBA/2J mice during acute ethanol withdrawal. Strain- and treatment-specific patterns of altered expression were observed for multiple genes associated with the Janus kinase/signal transducers and activators of transcription and the mitogen activated protein kinase pathways. Genes associated with both pathways are regulated in DBA/2J mice during chronic ethanol withdrawal, and to a lesser extent during acute ethanol withdrawal. Only those genes associated with the mitogen-activated protein kinase (MAPK) pathway exhibited changes in expression in C57BL/6J mice during ethanol withdrawal. Furthermore, genes associated with retinoic acid-mediated signaling show differential expression exclusively in C57BL/6J mice. These findings represent significant differences in cellular adaptation to ethanol between the DBA/2J and C57BL/6J strains.

Original languageEnglish (US)
Pages (from-to)35-45
Number of pages11
JournalGenes, Brain and Behavior
Volume1
Issue number1
DOIs
StatePublished - Jan 2002

Fingerprint

Ethanol
Inbred C57BL Mouse
Inbred DBA Mouse
Genes
Mitogen-Activated Protein Kinases
Janus Kinases
Gene Expression Regulation
Tretinoin
Oligonucleotide Array Sequence Analysis
Transducers
Transcriptome
Alcoholism
Hippocampus
Gene Expression

Keywords

  • C57BL/ 6J
  • DBA/ 2J
  • Ethanol
  • Gene expression
  • Microarray
  • Signal transduction
  • Withdrawal

ASJC Scopus subject areas

  • Neuroscience(all)
  • Genetics

Cite this

Expression profiling identifies strain-specific changes associated with ethanol withdrawal in mice. / Daniels, G. M.; Buck, Kari.

In: Genes, Brain and Behavior, Vol. 1, No. 1, 01.2002, p. 35-45.

Research output: Contribution to journalArticle

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