Expression profiles of cohesins, shugoshins and spindle assembly checkpoint genes in rhesus macaque oocytes predict their susceptibility for aneuploidy during embryonic development

Catherine Dupont, Alexandra J. Harvey, D. Randall Armant, Mary Zelinski, Carol A. Brenner

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    High frequencies of chromosomal anomalies are reported in human and non-human primate in vitro-produced preimplantation embryos. It is unclear why certain embryos develop aneuploidies while others remain euploid. A differential susceptibility to aneuploidy is most likely a consequence of events that occur before oocyte collection. One hypothesis is that the relative transcript levels of cohesins, shugoshins and spindle assembly checkpoint genes are correlated with the occurrence of chromosomal anomalies. Transcript levels of these genes were quantified in individual oocytes that were either mature (group 1, low aneuploidy rate) or immature (group 2, high aneuploidy rate) at retrieval, utilizing TaqMan-based real-time PCR. The transcript level in each oocyte was categorized as absent, below the median or above the median in order to conduct comparisons. Statistically significant differences were observed between group 1 and group 2 for SGOL1 and BUB1. There were more oocytes with SGOL1 expression levels above the median in group 1, while oocytes lacking BUB1 were only observed in group 1. These findings suggest that higher SGOL1 levels in group 1 oocytes could better protect against a premature separation of sister chromatids than in embryos derived from group 2 oocytes. The absence of BUB1 transcripts in group 1 was frequently associated with reduced expression of either mitotic cohesins or shugoshins. We hypothesize that ablation of BUB1 could induce mitotic arrest in oocytes that fail to express a complete complement of cohesins and shugoshins, thereby reducing the number of developing aneuploid preimplantation embryos.

    Original languageEnglish (US)
    Pages (from-to)740-748
    Number of pages9
    JournalCell Cycle
    Volume11
    Issue number4
    DOIs
    StatePublished - Feb 15 2012

    Fingerprint

    M Phase Cell Cycle Checkpoints
    Aneuploidy
    Macaca mulatta
    Oocytes
    Embryonic Development
    Genes
    Blastocyst
    Embryonic Structures
    Oocyte Retrieval
    Chromatids
    cohesins
    Primates
    Real-Time Polymerase Chain Reaction

    Keywords

    • Aneuploidy
    • Chromosomal abnormalities
    • Cohesin
    • Oocytes
    • Shugoshins
    • Spindle assembly checkpoint

    ASJC Scopus subject areas

    • Cell Biology
    • Molecular Biology
    • Developmental Biology

    Cite this

    Expression profiles of cohesins, shugoshins and spindle assembly checkpoint genes in rhesus macaque oocytes predict their susceptibility for aneuploidy during embryonic development. / Dupont, Catherine; Harvey, Alexandra J.; Armant, D. Randall; Zelinski, Mary; Brenner, Carol A.

    In: Cell Cycle, Vol. 11, No. 4, 15.02.2012, p. 740-748.

    Research output: Contribution to journalArticle

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