Expression pattern of bcar3, a downstream target of Gata2, and its binding partner, bcar1, during Xenopus development

Yangsook Song Green, Sunjong Kwon, Jan L. Christian

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Primitive hematopoiesis generates red blood cells that deliver oxygen to the developing embryo. Mesodermal cells commit to a primitive blood cell fate during gastrulation and, in order to do so the mesoderm must receive non-cell autonomous signals transmitted from other germ layers. In Xenopus, the transcription factor Gata2 functions in ectodermal cells to generate or transmit the non-cell autonomous signals. Here we have identified Breast Cancer Antiestrogen Resistance 3 (bcar3) as a gene that is induced in ectodermal cells downstream of Gata2. Bcar3 and its binding partner Bcar1 function to transduce integrin signaling, leading to changes in cellular morphology, motility and adhesion. We show that gata2, bcar3 and bcar1 are co-expressed in ventral ectoderm from early gastrula to early tailbud stages. At later stages of development, bcar3 and bcar1 are co-expressed in the spinal cord, notochord, fin mesenchyme and pronephros but each shows additional unique sites of expression. These co-expression and unique expression patterns suggest that Bcar3 and Bcar1 may function together but also independently during Xenopus development.

Original languageEnglish (US)
Pages (from-to)55-62
Number of pages8
JournalGene Expression Patterns
Volume20
Issue number1
DOIs
StatePublished - Jan 1 2016

Fingerprint

Estrogen Receptor Modulators
Xenopus
Mesoderm
Breast Neoplasms
Pronephros
Notochord
Gastrula
Germ Layers
Gastrulation
Ectoderm
Hematopoiesis
Integrins
Blood Cells
Spinal Cord
Transcription Factors
Embryonic Structures
Erythrocytes
Oxygen
Genes

Keywords

  • Bcar1
  • Bcar3
  • Ectoderm
  • Gastrulation
  • Gata2
  • Non-cell autonomous signals
  • Primitive hematopoiesis
  • Xenopus laevis

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Developmental Biology

Cite this

Expression pattern of bcar3, a downstream target of Gata2, and its binding partner, bcar1, during Xenopus development. / Green, Yangsook Song; Kwon, Sunjong; Christian, Jan L.

In: Gene Expression Patterns, Vol. 20, No. 1, 01.01.2016, p. 55-62.

Research output: Contribution to journalArticle

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