TY - JOUR
T1 - Expression pattern of bcar3, a downstream target of Gata2, and its binding partner, bcar1, during Xenopus development
AU - Green, Yangsook Song
AU - Kwon, Sunjong
AU - Christian, Jan L.
N1 - Publisher Copyright:
© 2015 Elsevier Ltd. All rights reserved.
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Primitive hematopoiesis generates red blood cells that deliver oxygen to the developing embryo. Mesodermal cells commit to a primitive blood cell fate during gastrulation and, in order to do so the mesoderm must receive non-cell autonomous signals transmitted from other germ layers. In Xenopus, the transcription factor Gata2 functions in ectodermal cells to generate or transmit the non-cell autonomous signals. Here we have identified Breast Cancer Antiestrogen Resistance 3 (bcar3) as a gene that is induced in ectodermal cells downstream of Gata2. Bcar3 and its binding partner Bcar1 function to transduce integrin signaling, leading to changes in cellular morphology, motility and adhesion. We show that gata2, bcar3 and bcar1 are co-expressed in ventral ectoderm from early gastrula to early tailbud stages. At later stages of development, bcar3 and bcar1 are co-expressed in the spinal cord, notochord, fin mesenchyme and pronephros but each shows additional unique sites of expression. These co-expression and unique expression patterns suggest that Bcar3 and Bcar1 may function together but also independently during Xenopus development.
AB - Primitive hematopoiesis generates red blood cells that deliver oxygen to the developing embryo. Mesodermal cells commit to a primitive blood cell fate during gastrulation and, in order to do so the mesoderm must receive non-cell autonomous signals transmitted from other germ layers. In Xenopus, the transcription factor Gata2 functions in ectodermal cells to generate or transmit the non-cell autonomous signals. Here we have identified Breast Cancer Antiestrogen Resistance 3 (bcar3) as a gene that is induced in ectodermal cells downstream of Gata2. Bcar3 and its binding partner Bcar1 function to transduce integrin signaling, leading to changes in cellular morphology, motility and adhesion. We show that gata2, bcar3 and bcar1 are co-expressed in ventral ectoderm from early gastrula to early tailbud stages. At later stages of development, bcar3 and bcar1 are co-expressed in the spinal cord, notochord, fin mesenchyme and pronephros but each shows additional unique sites of expression. These co-expression and unique expression patterns suggest that Bcar3 and Bcar1 may function together but also independently during Xenopus development.
KW - Bcar1
KW - Bcar3
KW - Ectoderm
KW - Gastrulation
KW - Gata2
KW - Non-cell autonomous signals
KW - Primitive hematopoiesis
KW - Xenopus laevis
UR - http://www.scopus.com/inward/record.url?scp=84955753372&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84955753372&partnerID=8YFLogxK
U2 - 10.1016/j.gep.2015.11.004
DO - 10.1016/j.gep.2015.11.004
M3 - Article
C2 - 26631802
AN - SCOPUS:84955753372
SN - 1567-133X
VL - 20
SP - 55
EP - 62
JO - Gene Expression Patterns
JF - Gene Expression Patterns
IS - 1
ER -