Expression of vascular endothelial growth factor and its receptors Flt-1 and KDR/Flk-1 in chronic cyclosporine nephrotoxicity

Fuad S. Shihab, William M. Bennett, Hong Yi, Takeshi F. Andoh

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Background. Vascular endothelial growth factor (VEGF) is an endothelial cell mitogen involved in angiogenesis, wound healing, and inflammation. Methods. Rats placed on low salt diet (LSD) or normal salt diet (NSD) were treated with cyclosporine (CsA) or vehicle (VH) and killed at 7 or 28 days. We studied the expression of VEGF and its receptors Flt-1 and KDR/Flk-1 mRNA by Northern and that of VEGF protein by Western blot. Results. CsA induced VEGF mRNA and protein expressions at 7 and 28 days in LSD rats. At 7 days, CsA up-regulated the expression of Flt-1 and KDR/Flk-1 receptors; however, at 28 days, Flt-1 remained unchanged whereas KDR/Flk-1 expression declined. In NSD rats, in which the lesion did not develop, the expression of VEGF and its receptors remained similar to control. Conclusions. What causes VEGF to be up-regulated remains unclear. Further studies are needed to study the role of hypoxia and other cytokines in relation to VEGF in this model.

Original languageEnglish (US)
Pages (from-to)164-168
Number of pages5
JournalTransplantation
Volume72
Issue number1
DOIs
StatePublished - Jul 15 2001
Externally publishedYes

ASJC Scopus subject areas

  • Transplantation

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