Expression of vascular endothelial growth factor and its receptors in rosacea

Justine R. Smith, Virginia B. Lanier, Rita Braziel, Katherine M. Falkenhagen, Clifton White, James (Jim) Rosenbaum

Research output: Contribution to journalArticle

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Abstract

Background: Rosacea is a common chronic disease of unclear pathogenesis, characterised by inflammation and vascular abnormalities of the facial skin and ocular surface. Recognising that vascular endothelial growth factor (VEGF) is vasoactive and has inflammatory activities, the expression of this molecule and its receptors, VEGF-R1 and VEGF-R2, in rosacea was investigated. Methods: Formalin-fixed, paraffin wax-embedded sections of skin obtained from 20 patients with rosacea were immunostained to detect expression of VEGF, VEGF-R1 and VEGF-R2, using an indirect methodology incorporating antigen retrieval. Adjacent sections were stained with haematoxylin and eosin. Results: Biopsy specimens were characterised by perivascular and perifollicular lymphohistiocytic infiltration and dilated vascular channels. In addition to keratinocyte and epithelial staining, which was also noted in normal skin, vascular endothelium frequently stained positive for VEGF-R1 (14/20, 70%) and VEGF-R2 (20/20, 100%), but infrequently for VEGF (2/20, 10%). In most specimens, infiltrating leucocytes, including lymphocytes, macrophages and plasma cells, expressed VEGF (17/20, 85%), VEGF-R1 (20/20, 100%) and VEGF-R2 (20/20, 100%). Conclusion: Expression of VEGF receptors, both by vascular endothelium and infiltrating mononuclear cells, is observed in rosacea. Although not expressed by endothelium, VEGF is present in epidermis and epithelium, and is expressed by infiltrating cells. VEGF receptor-ligand binding may contribute to the vascular changes and cellular infiltration that occurs in rosacea.

Original languageEnglish (US)
Pages (from-to)226-229
Number of pages4
JournalBritish Journal of Ophthalmology
Volume91
Issue number2
DOIs
StatePublished - Feb 2007

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Rosacea
Vascular Endothelial Growth Factor Receptor
Vascular Endothelial Growth Factor A
Blood Vessels
Vascular Endothelium
Skin Abnormalities
Skin
Waxes
Hematoxylin
Eosine Yellowish-(YS)
Plasma Cells
Keratinocytes
Epidermis
Paraffin
Formaldehyde
Endothelium

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Expression of vascular endothelial growth factor and its receptors in rosacea. / Smith, Justine R.; Lanier, Virginia B.; Braziel, Rita; Falkenhagen, Katherine M.; White, Clifton; Rosenbaum, James (Jim).

In: British Journal of Ophthalmology, Vol. 91, No. 2, 02.2007, p. 226-229.

Research output: Contribution to journalArticle

Smith, Justine R. ; Lanier, Virginia B. ; Braziel, Rita ; Falkenhagen, Katherine M. ; White, Clifton ; Rosenbaum, James (Jim). / Expression of vascular endothelial growth factor and its receptors in rosacea. In: British Journal of Ophthalmology. 2007 ; Vol. 91, No. 2. pp. 226-229.
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abstract = "Background: Rosacea is a common chronic disease of unclear pathogenesis, characterised by inflammation and vascular abnormalities of the facial skin and ocular surface. Recognising that vascular endothelial growth factor (VEGF) is vasoactive and has inflammatory activities, the expression of this molecule and its receptors, VEGF-R1 and VEGF-R2, in rosacea was investigated. Methods: Formalin-fixed, paraffin wax-embedded sections of skin obtained from 20 patients with rosacea were immunostained to detect expression of VEGF, VEGF-R1 and VEGF-R2, using an indirect methodology incorporating antigen retrieval. Adjacent sections were stained with haematoxylin and eosin. Results: Biopsy specimens were characterised by perivascular and perifollicular lymphohistiocytic infiltration and dilated vascular channels. In addition to keratinocyte and epithelial staining, which was also noted in normal skin, vascular endothelium frequently stained positive for VEGF-R1 (14/20, 70{\%}) and VEGF-R2 (20/20, 100{\%}), but infrequently for VEGF (2/20, 10{\%}). In most specimens, infiltrating leucocytes, including lymphocytes, macrophages and plasma cells, expressed VEGF (17/20, 85{\%}), VEGF-R1 (20/20, 100{\%}) and VEGF-R2 (20/20, 100{\%}). Conclusion: Expression of VEGF receptors, both by vascular endothelium and infiltrating mononuclear cells, is observed in rosacea. Although not expressed by endothelium, VEGF is present in epidermis and epithelium, and is expressed by infiltrating cells. VEGF receptor-ligand binding may contribute to the vascular changes and cellular infiltration that occurs in rosacea.",
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