Expression of Trk A receptors in the mammalian inner ear

C. F. Dai, Peter Steyger, Z. M. Wang, Z. Vass, Alfred Nuttall

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Tyrosine kinase receptors, including Trk A, Trk B and Trk C, participate in many different biological processes that are regulated by neurotrophic factors. Nerve growth factor (NGF)-triggered Trk A signaling is involved in growth, survival and differentiation of neurons in the central nervous system and in neural crest-derived cells. Trk A, Trk B and Trk C expression has been reported in the rat ventral cochlear nucleus. In the present study, we explored the immunocytochemical distribution of Trk A in the rodent inner ear. Rat and mouse cochleae were immunolabeled with a rabbit anti-Trk A polyclonal antibody (Chemicon) that has no reported cross-reactivity with Trk B and Trk C. In embryonic day 16 mice, no Trk A immunolabeling could be detected in the developing neuroepithelium. At postnatal day 6, weak Trk A labeling could be observed in both inner and outer hair cells. At postnatal day 12, enhanced punctate Trk A immunoexpression was present in hair cells. In adult mice and rats, intense Trk A labeling was observed in outer and inner hair cell bodies, in supporting cell bodies throughout the cochlea, and in spiral ganglion neurons. Trk A was not observed in stria vascularis, hair cell stereocilia, nor in the Trk B- and Trk C-rich cerebellum. This distribution pattern of Trk A suggests that its ligand, NGF, exerts significant trophic effects in the rodent inner ear.

Original languageEnglish (US)
Pages (from-to)1-11
Number of pages11
JournalHearing Research
Volume187
Issue number1-2
DOIs
StatePublished - Jan 2004

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Outer Auditory Hair Cells
Inner Auditory Hair Cells
Inner Ear
Cochlea
Nerve Growth Factor
Rodentia
Stereocilia
Stria Vascularis
Spiral Ganglion
Biological Phenomena
Cochlear Nucleus
Neurons
Neural Crest
Nerve Growth Factors
Receptor Protein-Tyrosine Kinases
Cerebellum
Central Nervous System
Rabbits
Ligands
Antibodies

ASJC Scopus subject areas

  • Sensory Systems

Cite this

Expression of Trk A receptors in the mammalian inner ear. / Dai, C. F.; Steyger, Peter; Wang, Z. M.; Vass, Z.; Nuttall, Alfred.

In: Hearing Research, Vol. 187, No. 1-2, 01.2004, p. 1-11.

Research output: Contribution to journalArticle

Dai, C. F. ; Steyger, Peter ; Wang, Z. M. ; Vass, Z. ; Nuttall, Alfred. / Expression of Trk A receptors in the mammalian inner ear. In: Hearing Research. 2004 ; Vol. 187, No. 1-2. pp. 1-11.
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