Expression of Transforming Growth Factor β1 in Patients with and without Previous Abdominal Surgery

Kristina G. Hobson, Michelle DeWing, Hung S. Ho, Bruce Wolfe, Kiho Cho, David G. Greenhalgh

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Hypothesis: Transforming growth factor β1 (TGF-β1) plays an important role in the formation of adhesions after abdominal operations. Design: Prospective, observational study. Setting: University-based, tertiary referral center. Patients: Patients undergoing elective open abdominal operations were recruited and divided into 2 groups. Twenty-two patients with a history of abdominal surgery were designated as study patients, and 10 patients with no history of abdominal surgery served as controls. Interventions: Samples of normal peritoneum, peritoneal scar tissues, and serum were obtained from all patients at the time of surgery. Main Outcome Measures: Samples were assayed for total TGF-β1 expression using an enzyme-linked immunosorbent assay. Results: Scar tissues expressed significantly greater amounts of TGF-β1 (0.47 pg/μL) compared with normal peritoneal tissue from both study patients (0.29 pg/ μL; P=.03) and controls (0.17 pg/μL; P=.002). Serum TGF-β1 levels were also higher in study patients (1.71 pg/μL) compared with controls (1.22 pg/μL; P=.02). Neither adhesion nor serum TGF-β1 expression correlated with time since last operation, total number of previous operations, or severity of intra-abdominal adhesions. Conclusion: These results suggest that TGF-β1 may play an important role in human peritoneal adhesion formation.

Original languageEnglish (US)
Pages (from-to)1249-1252
Number of pages4
JournalArchives of Surgery
Volume138
Issue number11
DOIs
StatePublished - Nov 2003
Externally publishedYes

Fingerprint

Transforming Growth Factors
Cicatrix
Serum
Peritoneum
Tertiary Care Centers
Observational Studies
Enzyme-Linked Immunosorbent Assay
Outcome Assessment (Health Care)
Prospective Studies

ASJC Scopus subject areas

  • Surgery

Cite this

Expression of Transforming Growth Factor β1 in Patients with and without Previous Abdominal Surgery. / Hobson, Kristina G.; DeWing, Michelle; Ho, Hung S.; Wolfe, Bruce; Cho, Kiho; Greenhalgh, David G.

In: Archives of Surgery, Vol. 138, No. 11, 11.2003, p. 1249-1252.

Research output: Contribution to journalArticle

Hobson, Kristina G. ; DeWing, Michelle ; Ho, Hung S. ; Wolfe, Bruce ; Cho, Kiho ; Greenhalgh, David G. / Expression of Transforming Growth Factor β1 in Patients with and without Previous Abdominal Surgery. In: Archives of Surgery. 2003 ; Vol. 138, No. 11. pp. 1249-1252.
@article{0e654ac4898446b9b21f92b6c2d9e7ef,
title = "Expression of Transforming Growth Factor β1 in Patients with and without Previous Abdominal Surgery",
abstract = "Hypothesis: Transforming growth factor β1 (TGF-β1) plays an important role in the formation of adhesions after abdominal operations. Design: Prospective, observational study. Setting: University-based, tertiary referral center. Patients: Patients undergoing elective open abdominal operations were recruited and divided into 2 groups. Twenty-two patients with a history of abdominal surgery were designated as study patients, and 10 patients with no history of abdominal surgery served as controls. Interventions: Samples of normal peritoneum, peritoneal scar tissues, and serum were obtained from all patients at the time of surgery. Main Outcome Measures: Samples were assayed for total TGF-β1 expression using an enzyme-linked immunosorbent assay. Results: Scar tissues expressed significantly greater amounts of TGF-β1 (0.47 pg/μL) compared with normal peritoneal tissue from both study patients (0.29 pg/ μL; P=.03) and controls (0.17 pg/μL; P=.002). Serum TGF-β1 levels were also higher in study patients (1.71 pg/μL) compared with controls (1.22 pg/μL; P=.02). Neither adhesion nor serum TGF-β1 expression correlated with time since last operation, total number of previous operations, or severity of intra-abdominal adhesions. Conclusion: These results suggest that TGF-β1 may play an important role in human peritoneal adhesion formation.",
author = "Hobson, {Kristina G.} and Michelle DeWing and Ho, {Hung S.} and Bruce Wolfe and Kiho Cho and Greenhalgh, {David G.}",
year = "2003",
month = "11",
doi = "10.1001/archsurg.138.11.1249",
language = "English (US)",
volume = "138",
pages = "1249--1252",
journal = "JAMA Surgery",
issn = "2168-6254",
publisher = "American Medical Association",
number = "11",

}

TY - JOUR

T1 - Expression of Transforming Growth Factor β1 in Patients with and without Previous Abdominal Surgery

AU - Hobson, Kristina G.

AU - DeWing, Michelle

AU - Ho, Hung S.

AU - Wolfe, Bruce

AU - Cho, Kiho

AU - Greenhalgh, David G.

PY - 2003/11

Y1 - 2003/11

N2 - Hypothesis: Transforming growth factor β1 (TGF-β1) plays an important role in the formation of adhesions after abdominal operations. Design: Prospective, observational study. Setting: University-based, tertiary referral center. Patients: Patients undergoing elective open abdominal operations were recruited and divided into 2 groups. Twenty-two patients with a history of abdominal surgery were designated as study patients, and 10 patients with no history of abdominal surgery served as controls. Interventions: Samples of normal peritoneum, peritoneal scar tissues, and serum were obtained from all patients at the time of surgery. Main Outcome Measures: Samples were assayed for total TGF-β1 expression using an enzyme-linked immunosorbent assay. Results: Scar tissues expressed significantly greater amounts of TGF-β1 (0.47 pg/μL) compared with normal peritoneal tissue from both study patients (0.29 pg/ μL; P=.03) and controls (0.17 pg/μL; P=.002). Serum TGF-β1 levels were also higher in study patients (1.71 pg/μL) compared with controls (1.22 pg/μL; P=.02). Neither adhesion nor serum TGF-β1 expression correlated with time since last operation, total number of previous operations, or severity of intra-abdominal adhesions. Conclusion: These results suggest that TGF-β1 may play an important role in human peritoneal adhesion formation.

AB - Hypothesis: Transforming growth factor β1 (TGF-β1) plays an important role in the formation of adhesions after abdominal operations. Design: Prospective, observational study. Setting: University-based, tertiary referral center. Patients: Patients undergoing elective open abdominal operations were recruited and divided into 2 groups. Twenty-two patients with a history of abdominal surgery were designated as study patients, and 10 patients with no history of abdominal surgery served as controls. Interventions: Samples of normal peritoneum, peritoneal scar tissues, and serum were obtained from all patients at the time of surgery. Main Outcome Measures: Samples were assayed for total TGF-β1 expression using an enzyme-linked immunosorbent assay. Results: Scar tissues expressed significantly greater amounts of TGF-β1 (0.47 pg/μL) compared with normal peritoneal tissue from both study patients (0.29 pg/ μL; P=.03) and controls (0.17 pg/μL; P=.002). Serum TGF-β1 levels were also higher in study patients (1.71 pg/μL) compared with controls (1.22 pg/μL; P=.02). Neither adhesion nor serum TGF-β1 expression correlated with time since last operation, total number of previous operations, or severity of intra-abdominal adhesions. Conclusion: These results suggest that TGF-β1 may play an important role in human peritoneal adhesion formation.

UR - http://www.scopus.com/inward/record.url?scp=0242695752&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0242695752&partnerID=8YFLogxK

U2 - 10.1001/archsurg.138.11.1249

DO - 10.1001/archsurg.138.11.1249

M3 - Article

C2 - 14609876

AN - SCOPUS:0242695752

VL - 138

SP - 1249

EP - 1252

JO - JAMA Surgery

JF - JAMA Surgery

SN - 2168-6254

IS - 11

ER -