Expression of the thiazide-sensitive Na-Cl cotransporter by rabbit distal convoluted tubule cells

S. Bachmann, H. Velazquez, N. Obermuller, R. F. Reilly, D. Moser, David Ellison

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

A thiazide-sensitive Na-Cl cotransporter contributes importantly to mammalian salt homeostasis by mediating Na-Cl transport along the renal distal tubule. Although it has been accepted that thiazide-sensitive Na-Cl cotransport occurs predominantly along the distal convoluted tubule in rats and mice, sites of expression in the rabbit have been controversial. A commonly accepted model of rabbit distal nephron transport pathways identifies the connecting tubule, not the distal convoluted tubule, as the predominant site of thiazide-sensitive Na-Cl cotransport. The thiazide- sensitive Na-Cl cotransporter has been cloned recently. The present experiments were designed to localize sites of thiazide-sensitive Na-Cl cotransporter mRNA expression along the rabbit distal nephron. Nonradioactive in situ hybridization with a thiazide-sensitive Na-Cl cotransporter probe was combined with immunocytochemistry with an antibody that recognizes distal convoluted tubule cells and with a Na+/Ca2+ exchanger antibody that recognizes only connecting tubule cells. The results indicate that thiazide- sensitive Na-Cl cotransporter mRNA is highly expressed by cells of the distal convoluted tubule and not by connecting tubule cells. Segments that stain with the Na+/Ca2+ exchanger antibody (connecting tubules) do not demonstrate thiazide-sensitive Na-Cl cotransporter mRNA expression. We conclude that the predominant site of thiazide-sensitive Na-Cl cotransporter mRNA expression in rabbit distal nephron is the distal convoluted tubule and that sites of mRNA expression of electroneutral Na and Cl transport are similar in rabbits, rats, and mice.

Original languageEnglish (US)
Pages (from-to)2510-2514
Number of pages5
JournalJournal of Clinical Investigation
Volume96
Issue number5
StatePublished - 1995
Externally publishedYes

Fingerprint

Rabbits
Nephrons
Messenger RNA
Thiazides
Antibodies
Distal Kidney Tubule
thiazide receptor
In Situ Hybridization
Homeostasis
Coloring Agents
Salts
Immunohistochemistry

Keywords

  • calcium transport proteins
  • diuretics, thiazide
  • in situ hybridization
  • kidney tubules, distal
  • sodium chloride

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Bachmann, S., Velazquez, H., Obermuller, N., Reilly, R. F., Moser, D., & Ellison, D. (1995). Expression of the thiazide-sensitive Na-Cl cotransporter by rabbit distal convoluted tubule cells. Journal of Clinical Investigation, 96(5), 2510-2514.

Expression of the thiazide-sensitive Na-Cl cotransporter by rabbit distal convoluted tubule cells. / Bachmann, S.; Velazquez, H.; Obermuller, N.; Reilly, R. F.; Moser, D.; Ellison, David.

In: Journal of Clinical Investigation, Vol. 96, No. 5, 1995, p. 2510-2514.

Research output: Contribution to journalArticle

Bachmann, S, Velazquez, H, Obermuller, N, Reilly, RF, Moser, D & Ellison, D 1995, 'Expression of the thiazide-sensitive Na-Cl cotransporter by rabbit distal convoluted tubule cells', Journal of Clinical Investigation, vol. 96, no. 5, pp. 2510-2514.
Bachmann, S. ; Velazquez, H. ; Obermuller, N. ; Reilly, R. F. ; Moser, D. ; Ellison, David. / Expression of the thiazide-sensitive Na-Cl cotransporter by rabbit distal convoluted tubule cells. In: Journal of Clinical Investigation. 1995 ; Vol. 96, No. 5. pp. 2510-2514.
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