Expression of the hereditary hemochromatosis protein HFE increases ferritin levels by inhibiting iron export in HT29 cells

Paige Davies, Caroline Enns

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Iron is essential for life in almost all organisms and, in mammals, is absorbed through the villus cells of the duodenum. Using a human colonic carcinoma cell line that has many duodenal characteristics, HT29, we show that genes involved in intestinal iron transport are endogenously expressed. When stably transfected to express the hereditary hemochromatosis protein HFE these cells have increased ferritin levels. We demonstrate that this is not due to an effect on the transferrin (TF)-mediated iron uptake pathway but rather due to inhibition of iron efflux from the cell. The effect of HFE was independent of its interaction with TF receptor 1 as indicated by similar results using both the wild type HFE and the W81A mutant that binds TF receptor 1 with greatly reduced affinity. HFE expression did not affect the mRNA levels of most of the genes involved in iron absorption that were tested; however, it did correspond to a decrease in hephaestin message levels. These results point to a role for HFE in inhibition of iron efflux in HT29 cells. This is a distinct role from that in HeLa and human embryonic kidney 293 cells where HFE has been shown to inhibit TF-mediated iron uptake resulting in decreased ferritin levels. Such a distinction suggests a multifunctional role for HFE that is dependent upon expression levels of proteins involved in iron transport.

Original languageEnglish (US)
Pages (from-to)25085-25092
Number of pages8
JournalJournal of Biological Chemistry
Volume279
Issue number24
DOIs
StatePublished - Jun 11 2004

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HT29 Cells
Ferritins
Iron
Proteins
Transferrin Receptors
Transferrin
Genes
Mammals
Hemochromatosis Protein
Duodenum
Cells
Carcinoma
Kidney
Cell Line
Messenger RNA

ASJC Scopus subject areas

  • Biochemistry

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Expression of the hereditary hemochromatosis protein HFE increases ferritin levels by inhibiting iron export in HT29 cells. / Davies, Paige; Enns, Caroline.

In: Journal of Biological Chemistry, Vol. 279, No. 24, 11.06.2004, p. 25085-25092.

Research output: Contribution to journalArticle

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