Expression of the gibbon ape leukemia virus receptor-1 in rhesus macaque tissues

Scott W. Wong, Ryan M. Swanson, Eric P. Bergquam

Research output: Contribution to journalArticle

Abstract

A cDNA fragment specific for the rhesus macaque gibbon ape leukemia virus (GaLV) receptor (Glvr-1) was isolated by polymerase chain reaction (PCR) amplification and oligonucleotide primers specific for human Glvr-1 and a cDNA library derived from rhesus macaque brain. Sequence analysis of the fragment revealed the polypeptide domain necessary for infection by GaLV. This fragment was utilized to elucidate expression levels of Glvr-1 RNA in rhesus macaque tissues. By Northern blot analysis, Glvr-1 RNA is most abundantly expressed in the thymus and bone marrow, with detectable levels also in the brain and testes of juvenile male macaques. In the developing 70-days gestation fetus, Glvr-1 expression was observed predominately in the liver and spleen. Although additional studies are required, these studies support the notion that cell types involved in hematopoiesis express Glvr-1.

Original languageEnglish (US)
Pages (from-to)59-62
Number of pages4
JournalJournal of medical primatology
Volume26
Issue number1-2
DOIs
StatePublished - Jan 1 1997

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Keywords

  • Gene therapy
  • Retrovirus vector

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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