Expression of tachykinins in nonnociceptive vagal afferent neurons during respiratory viral infection in guinea pigs

Michael J. Carr, Dawn D. Hunter, David Jacoby, Bradley J. Undem

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

Immunohistochemistry was combined with retrograde labeling to characterize the effect of respiratory infection with Sendai virus on the number of Substance P/Neurokinin A-containing vagal afferent neurons whose cell bodies resided in the nodose ganglia and whose receptive fields were located in guinea pig trachea. Of the neurons labeled from the trachea of vehicle-inoculated guinea pigs, few stained positively for Substance P/Neurokinin A (∼ 3% of total labeled neurons). These neurons had small diameter cell bodies (mode = 16-20 μm), a feature of nociceptive-like C-fibers. Viral infection (Day 4 after inoculation) was associated with a significantly greater number of labeled neurons containing Substance P/Neurokinin A (∼ 20% of total labeled neurons). The majority of these had a relatively large cell body diameter (mode = 36-40 μm), a feature of nonnociceptive afferent neurons. This induction appeared to be reversible as there were significantly fewer Substance P/Neurokinin A positive neurons in nodose ganglia from virus-inoculated guinea pigs at Day 28 after inoculation, a time point when virus-induced airway inflammation had all but resolved. These findings support the hypothesis that viral infection leads to a qualitative change in the vagal afferent innervation of guinea pig airways such that both small diameter nociceptive-like neurons and large diameter nonnociceptive neurons express tachykinins.

Original languageEnglish (US)
Pages (from-to)1071-1075
Number of pages5
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume165
Issue number8
StatePublished - Apr 15 2002
Externally publishedYes

Fingerprint

Afferent Neurons
Tachykinins
Virus Diseases
Respiratory Tract Infections
Guinea Pigs
Neurokinin A
Neurons
Substance P
Nodose Ganglion
Trachea
Viruses
Sendai virus
Unmyelinated Nerve Fibers
Nociceptors
Immunohistochemistry
Inflammation

Keywords

  • Airway innervation
  • Neurokinin A
  • Respiratory viral infection
  • Sensory nerves
  • Substance P
  • Tachykinins

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Expression of tachykinins in nonnociceptive vagal afferent neurons during respiratory viral infection in guinea pigs. / Carr, Michael J.; Hunter, Dawn D.; Jacoby, David; Undem, Bradley J.

In: American Journal of Respiratory and Critical Care Medicine, Vol. 165, No. 8, 15.04.2002, p. 1071-1075.

Research output: Contribution to journalArticle

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