Expression of sialyl-lewis X, an E-selectin ligand, in inflammation, immune processes, and lymphoid tissues

J. Michael Munro, Siu K. Lo, Christopher Corless, Michael J. Robertson, Nina C. Lee, Raymond L. Barnhill, David S. Weinberg, Michael P. Bevilacqua

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

The carbohydrate structure sialyl-Lewis X (SLex) can function as a ligand for E-selectin, formerly known as endothelial leukocyte adhesion molecule-1 (ELAM-1). This study was performed to analyze the expression of SLex by leukocytes and other cell types in the context of inflammatory and immune processes. Human peripheral blood cells were examined by flow cytometry using monoclonal antibody CSLEX1 directed against SLex. Cell surface SLex was found in abundance on nearly all isolated polymorphonuclear leukocytes (PMN) and monocytes, and at low levels on a substantial portion (up to 40%) of natural killer cells. This moiety was expressed also on approximately 10% of peripheral blood T cells, Immunohistochemistry was performed on various human tissues involved in inflammatory or immune processes and on secondary lymphoid tissues. In acute appendicitis, endothelial cells of postcapillary venules expressed E-selectin, and most PMN, both within vessels and extravasated, expressed SLex. A substantial number of monocytes/macrophages in inflamed appendiceal, synovial, and dermal tissues also reacted with antibody CSLEX1; however, only rare tissue macrophages in uninflamed nonlymphoid sites showed expression of SLex. These observations are consistent with the concept that SLex on circulating PMN and monocytes functions as a ligand for endothelial E-selectin in the development of inflammatory reactions. SLex-positive lymphocytes also were seen, notably, T lymphocytes in inflamed skin. An unexpected finding was that the CSLEX1 antibody also reacted with venular endothelium in certain lymphoid tissues and in inflamed appendix, but not with endothelium in normal appendix. Whether the SLex antigen identified on endothelium represents de novo expression or passive adsorption remains to be determined.

Original languageEnglish (US)
Pages (from-to)1397-1408
Number of pages12
JournalAmerican Journal of Pathology
Volume141
Issue number6
StatePublished - Dec 1992
Externally publishedYes

Fingerprint

E-Selectin
Lymphoid Tissue
Ligands
Inflammation
Endothelium
Monocytes
Neutrophils
Blood Cells
Macrophages
T-Lymphocytes
Skin
Venules
Antibodies
Appendicitis
Natural Killer Cells
Adsorption
Flow Cytometry
Leukocytes
Endothelial Cells
Immunohistochemistry

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Munro, J. M., Lo, S. K., Corless, C., Robertson, M. J., Lee, N. C., Barnhill, R. L., ... Bevilacqua, M. P. (1992). Expression of sialyl-lewis X, an E-selectin ligand, in inflammation, immune processes, and lymphoid tissues. American Journal of Pathology, 141(6), 1397-1408.

Expression of sialyl-lewis X, an E-selectin ligand, in inflammation, immune processes, and lymphoid tissues. / Munro, J. Michael; Lo, Siu K.; Corless, Christopher; Robertson, Michael J.; Lee, Nina C.; Barnhill, Raymond L.; Weinberg, David S.; Bevilacqua, Michael P.

In: American Journal of Pathology, Vol. 141, No. 6, 12.1992, p. 1397-1408.

Research output: Contribution to journalArticle

Munro, JM, Lo, SK, Corless, C, Robertson, MJ, Lee, NC, Barnhill, RL, Weinberg, DS & Bevilacqua, MP 1992, 'Expression of sialyl-lewis X, an E-selectin ligand, in inflammation, immune processes, and lymphoid tissues', American Journal of Pathology, vol. 141, no. 6, pp. 1397-1408.
Munro, J. Michael ; Lo, Siu K. ; Corless, Christopher ; Robertson, Michael J. ; Lee, Nina C. ; Barnhill, Raymond L. ; Weinberg, David S. ; Bevilacqua, Michael P. / Expression of sialyl-lewis X, an E-selectin ligand, in inflammation, immune processes, and lymphoid tissues. In: American Journal of Pathology. 1992 ; Vol. 141, No. 6. pp. 1397-1408.
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abstract = "The carbohydrate structure sialyl-Lewis X (SLex) can function as a ligand for E-selectin, formerly known as endothelial leukocyte adhesion molecule-1 (ELAM-1). This study was performed to analyze the expression of SLex by leukocytes and other cell types in the context of inflammatory and immune processes. Human peripheral blood cells were examined by flow cytometry using monoclonal antibody CSLEX1 directed against SLex. Cell surface SLex was found in abundance on nearly all isolated polymorphonuclear leukocytes (PMN) and monocytes, and at low levels on a substantial portion (up to 40{\%}) of natural killer cells. This moiety was expressed also on approximately 10{\%} of peripheral blood T cells, Immunohistochemistry was performed on various human tissues involved in inflammatory or immune processes and on secondary lymphoid tissues. In acute appendicitis, endothelial cells of postcapillary venules expressed E-selectin, and most PMN, both within vessels and extravasated, expressed SLex. A substantial number of monocytes/macrophages in inflamed appendiceal, synovial, and dermal tissues also reacted with antibody CSLEX1; however, only rare tissue macrophages in uninflamed nonlymphoid sites showed expression of SLex. These observations are consistent with the concept that SLex on circulating PMN and monocytes functions as a ligand for endothelial E-selectin in the development of inflammatory reactions. SLex-positive lymphocytes also were seen, notably, T lymphocytes in inflamed skin. An unexpected finding was that the CSLEX1 antibody also reacted with venular endothelium in certain lymphoid tissues and in inflamed appendix, but not with endothelium in normal appendix. Whether the SLex antigen identified on endothelium represents de novo expression or passive adsorption remains to be determined.",
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