Expression of neurophysin-related precursor in cell membranes of a small-cell lung carcinoma

Lawrence C. Rosenbaum, Edward Neuwelt, Hubert H M Van Tol, Y. Peng Loh, Joseph G. Verbalis, Ingegerd Hellström, Karl Erik Hellström, Gajanan Nilaver

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A monoclonal antibody (mAb L6) to a small-cell lung carcinoma surface antigen recognizes a common epitope of vasopressin-neurophysin and oxytocin-neurophy sin in hypothalamic nuclei. We now report on the identification of a neurophysin-like precursor in human lung carcinoma (LX-1) cell membrane. mAb L6 immunoaffinity chromatography of solubilized membranes resulted in a single band of ≈45 kDa. Western blot analysis demonstrated immunoreactivity of this band with mAb L6, anti-vasopressin, and an antibody to the vasopressin precursor, pro-pressophysin. N-terminal sequencing of this band demonstrated a 21-amino acid homology with the N terminus of human pro-pressophysin, and substitution of a Cys33 residue in the tumor antigen with Arg33. Absence of immunoreactivity with the antibodies described above in cytosolic extracts and culture medium suggests nonsecretion of processed or intact pro-pressophysin-like peptide. Northern analysis of LX-1 mRNA with a 30-mer to the C terminus of rat pro-pressophysin resulted in a band of ≈1000 base pairs, 250 base pairs larger than hypothalamic message. In situ hybridization of LX-1 tumor-bearing nude rat brain with the same probe demonstrated specific hybridization in rat hypothalamus and xenografted tumor. These findings suggest expression of a pro-pressophysin-like protein in this tumor cell line that is preferentially targeted to the cell membrane.

Original languageEnglish (US)
Pages (from-to)9928-9932
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number24
StatePublished - 1990

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Neurophysins
Small Cell Lung Carcinoma
Vasopressins
Cell Membrane
Base Pairing
Hypothalamic Neoplasms
Nude Rats
Neoplasm Antigens
Oxytocin
Surface Antigens
Tumor Cell Line
In Situ Hybridization
Culture Media
Chromatography
Epitopes
Anti-Idiotypic Antibodies
Western Blotting
Monoclonal Antibodies
Carcinoma
Amino Acids

Keywords

  • In situ hybridization
  • Lung neoplasms
  • Northern blots
  • Tumor antigen
  • Western blots

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Rosenbaum, L. C., Neuwelt, E., Van Tol, H. H. M., Peng Loh, Y., Verbalis, J. G., Hellström, I., ... Nilaver, G. (1990). Expression of neurophysin-related precursor in cell membranes of a small-cell lung carcinoma. Proceedings of the National Academy of Sciences of the United States of America, 87(24), 9928-9932.

Expression of neurophysin-related precursor in cell membranes of a small-cell lung carcinoma. / Rosenbaum, Lawrence C.; Neuwelt, Edward; Van Tol, Hubert H M; Peng Loh, Y.; Verbalis, Joseph G.; Hellström, Ingegerd; Hellström, Karl Erik; Nilaver, Gajanan.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, No. 24, 1990, p. 9928-9932.

Research output: Contribution to journalArticle

Rosenbaum, LC, Neuwelt, E, Van Tol, HHM, Peng Loh, Y, Verbalis, JG, Hellström, I, Hellström, KE & Nilaver, G 1990, 'Expression of neurophysin-related precursor in cell membranes of a small-cell lung carcinoma', Proceedings of the National Academy of Sciences of the United States of America, vol. 87, no. 24, pp. 9928-9932.
Rosenbaum, Lawrence C. ; Neuwelt, Edward ; Van Tol, Hubert H M ; Peng Loh, Y. ; Verbalis, Joseph G. ; Hellström, Ingegerd ; Hellström, Karl Erik ; Nilaver, Gajanan. / Expression of neurophysin-related precursor in cell membranes of a small-cell lung carcinoma. In: Proceedings of the National Academy of Sciences of the United States of America. 1990 ; Vol. 87, No. 24. pp. 9928-9932.
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