Abstract
The cutaneous lymphocyte-associated antigen recognized by the monoclonal antibody HECA-452 has been thought to play a major role in the homing of memory T-cell subsets to the skin by virtue of its ability to bind to E-selectin of dermal microvascular endothelial cells. Considering that the homing of different leukocyte populations to the skin may involve similar mechanisms, we studied the expression of HECA-452-reactive molecules on CD1a+ epidermal Langerhans cells. Immunofluorescence double-labeling of cryostat sections and epidermal sheets of normal skin revealed HECA-452 immunoreactivity on a subpopulation of dermal and epidermal CD1a+ cells, whereas upon flow-cytometric analysis of epidermal single cell suspensions virtually all CD1a+ cells bound HECA-452 antibodies. We observed a marked upregulation of HECA-452-antigen expression on CD1a+ epidermal cells and a pronounced increase in the number of HECA-452+/CD1a+ dermal cells in lesional skin from inflammatory and neoplastic lymphocytic skin diseases, compared to normal skin. The molecule detected by the HECA-452 antibody on Langerhans cells is neuraminidase sensitive and contains a CD15 (Lewisx) carbohydrate backbone. Because Langerhans cells react with the sialyl-Lewisx-specific antibody CSLEX1, it is very likely that the HECA-452-reactive structure is or contains sialyl-Lewisx. Our data are compatible with the view that i) resident epidermal Langerhans cells upregulate HECA-452-antigen expression due to the cytokine profile generated in the disease process or ii) that Langerhans cell precursors express HECA-452-antigens and show an enhanced immigration into lesional skin. The characterization of HECA-452+ cells in peripheral blood may not only clarify this issue but may also help to identify the still elusive Langerhans cell-precursor.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 773-780 |
| Number of pages | 8 |
| Journal | Journal of Investigative Dermatology |
| Volume | 102 |
| Issue number | 5 |
| State | Published - May 1994 |
| Externally published | Yes |
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Keywords
- CD15
- sLe
ASJC Scopus subject areas
- Dermatology
Cite this
Expression of monoclonal antibody HECA-452 - defined E-selectin ligands on Langerhans cells in normal and diseased skin. / Koszik, Frieder; Strunk, Dirk; Simonitsch, Ingrid; Picker, Louis; Stingl, Georg; Payer, Elisabeth.
In: Journal of Investigative Dermatology, Vol. 102, No. 5, 05.1994, p. 773-780.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of monoclonal antibody HECA-452 - defined E-selectin ligands on Langerhans cells in normal and diseased skin
AU - Koszik, Frieder
AU - Strunk, Dirk
AU - Simonitsch, Ingrid
AU - Picker, Louis
AU - Stingl, Georg
AU - Payer, Elisabeth
PY - 1994/5
Y1 - 1994/5
N2 - The cutaneous lymphocyte-associated antigen recognized by the monoclonal antibody HECA-452 has been thought to play a major role in the homing of memory T-cell subsets to the skin by virtue of its ability to bind to E-selectin of dermal microvascular endothelial cells. Considering that the homing of different leukocyte populations to the skin may involve similar mechanisms, we studied the expression of HECA-452-reactive molecules on CD1a+ epidermal Langerhans cells. Immunofluorescence double-labeling of cryostat sections and epidermal sheets of normal skin revealed HECA-452 immunoreactivity on a subpopulation of dermal and epidermal CD1a+ cells, whereas upon flow-cytometric analysis of epidermal single cell suspensions virtually all CD1a+ cells bound HECA-452 antibodies. We observed a marked upregulation of HECA-452-antigen expression on CD1a+ epidermal cells and a pronounced increase in the number of HECA-452+/CD1a+ dermal cells in lesional skin from inflammatory and neoplastic lymphocytic skin diseases, compared to normal skin. The molecule detected by the HECA-452 antibody on Langerhans cells is neuraminidase sensitive and contains a CD15 (Lewisx) carbohydrate backbone. Because Langerhans cells react with the sialyl-Lewisx-specific antibody CSLEX1, it is very likely that the HECA-452-reactive structure is or contains sialyl-Lewisx. Our data are compatible with the view that i) resident epidermal Langerhans cells upregulate HECA-452-antigen expression due to the cytokine profile generated in the disease process or ii) that Langerhans cell precursors express HECA-452-antigens and show an enhanced immigration into lesional skin. The characterization of HECA-452+ cells in peripheral blood may not only clarify this issue but may also help to identify the still elusive Langerhans cell-precursor.
AB - The cutaneous lymphocyte-associated antigen recognized by the monoclonal antibody HECA-452 has been thought to play a major role in the homing of memory T-cell subsets to the skin by virtue of its ability to bind to E-selectin of dermal microvascular endothelial cells. Considering that the homing of different leukocyte populations to the skin may involve similar mechanisms, we studied the expression of HECA-452-reactive molecules on CD1a+ epidermal Langerhans cells. Immunofluorescence double-labeling of cryostat sections and epidermal sheets of normal skin revealed HECA-452 immunoreactivity on a subpopulation of dermal and epidermal CD1a+ cells, whereas upon flow-cytometric analysis of epidermal single cell suspensions virtually all CD1a+ cells bound HECA-452 antibodies. We observed a marked upregulation of HECA-452-antigen expression on CD1a+ epidermal cells and a pronounced increase in the number of HECA-452+/CD1a+ dermal cells in lesional skin from inflammatory and neoplastic lymphocytic skin diseases, compared to normal skin. The molecule detected by the HECA-452 antibody on Langerhans cells is neuraminidase sensitive and contains a CD15 (Lewisx) carbohydrate backbone. Because Langerhans cells react with the sialyl-Lewisx-specific antibody CSLEX1, it is very likely that the HECA-452-reactive structure is or contains sialyl-Lewisx. Our data are compatible with the view that i) resident epidermal Langerhans cells upregulate HECA-452-antigen expression due to the cytokine profile generated in the disease process or ii) that Langerhans cell precursors express HECA-452-antigens and show an enhanced immigration into lesional skin. The characterization of HECA-452+ cells in peripheral blood may not only clarify this issue but may also help to identify the still elusive Langerhans cell-precursor.
KW - CD15
KW - sLe
UR - http://www.scopus.com/inward/record.url?scp=0028347425&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028347425&partnerID=8YFLogxK
M3 - Article
VL - 102
SP - 773
EP - 780
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
SN - 0022-202X
IS - 5
ER -