Expression of monoclonal antibody HECA-452 - defined E-selectin ligands on Langerhans cells in normal and diseased skin

Frieder Koszik, Dirk Strunk, Ingrid Simonitsch, Louis Picker, Georg Stingl, Elisabeth Payer

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The cutaneous lymphocyte-associated antigen recognized by the monoclonal antibody HECA-452 has been thought to play a major role in the homing of memory T-cell subsets to the skin by virtue of its ability to bind to E-selectin of dermal microvascular endothelial cells. Considering that the homing of different leukocyte populations to the skin may involve similar mechanisms, we studied the expression of HECA-452-reactive molecules on CD1a+ epidermal Langerhans cells. Immunofluorescence double-labeling of cryostat sections and epidermal sheets of normal skin revealed HECA-452 immunoreactivity on a subpopulation of dermal and epidermal CD1a+ cells, whereas upon flow-cytometric analysis of epidermal single cell suspensions virtually all CD1a+ cells bound HECA-452 antibodies. We observed a marked upregulation of HECA-452-antigen expression on CD1a+ epidermal cells and a pronounced increase in the number of HECA-452+/CD1a+ dermal cells in lesional skin from inflammatory and neoplastic lymphocytic skin diseases, compared to normal skin. The molecule detected by the HECA-452 antibody on Langerhans cells is neuraminidase sensitive and contains a CD15 (Lewisx) carbohydrate backbone. Because Langerhans cells react with the sialyl-Lewisx-specific antibody CSLEX1, it is very likely that the HECA-452-reactive structure is or contains sialyl-Lewisx. Our data are compatible with the view that i) resident epidermal Langerhans cells upregulate HECA-452-antigen expression due to the cytokine profile generated in the disease process or ii) that Langerhans cell precursors express HECA-452-antigens and show an enhanced immigration into lesional skin. The characterization of HECA-452+ cells in peripheral blood may not only clarify this issue but may also help to identify the still elusive Langerhans cell-precursor.

Original languageEnglish (US)
Pages (from-to)773-780
Number of pages8
JournalJournal of Investigative Dermatology
Volume102
Issue number5
StatePublished - May 1994
Externally publishedYes

Fingerprint

E-Selectin
Langerhans Cells
Skin Diseases
Skin
Monoclonal Antibodies
Ligands
Antigens
Antibodies
Molecules
Cryostats
T-cells
Lymphocytes
Up-Regulation
Endothelial cells
Neuraminidase
Single-Cell Analysis
Labeling
Suspensions
Blood
Emigration and Immigration

Keywords

  • CD15
  • sLe

ASJC Scopus subject areas

  • Dermatology

Cite this

Expression of monoclonal antibody HECA-452 - defined E-selectin ligands on Langerhans cells in normal and diseased skin. / Koszik, Frieder; Strunk, Dirk; Simonitsch, Ingrid; Picker, Louis; Stingl, Georg; Payer, Elisabeth.

In: Journal of Investigative Dermatology, Vol. 102, No. 5, 05.1994, p. 773-780.

Research output: Contribution to journalArticle

Koszik, Frieder ; Strunk, Dirk ; Simonitsch, Ingrid ; Picker, Louis ; Stingl, Georg ; Payer, Elisabeth. / Expression of monoclonal antibody HECA-452 - defined E-selectin ligands on Langerhans cells in normal and diseased skin. In: Journal of Investigative Dermatology. 1994 ; Vol. 102, No. 5. pp. 773-780.
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