Expression of matrix metalloproteinases and inhibitor by human trabecular meshwork

J. P. Alexander, J. R. Samples, E. M. Van Buskirk, Ted Acott

Research output: Contribution to journalArticle

165 Citations (Scopus)

Abstract

Extracellular matrix (ECM) turnover and remodeling are initiated, at least in part, by the regulated secretion of members of a family of matrix metalloproteinases. Human and bovine trabecular meshwork in culture secrete interstitial collagenase, both the 72- and the 92-kD forms of type IV collagenase (gelatinases) and stromelysin, and the tissue inhibitor of metalloproteinases (TIMP). These proteinases and TIMP were identified by immunoblotting western transfers from sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using several specific antiprotein and antipeptide polyclonal antibodies. Gelatinase and stromelysin enzymatic activities were also analyzed by substrate SDS-PAGE, in which proteinase substrates were polymerized into the gels before electrophoresis to allow subsequent activity assays. These matrix metalloproteinases and TIMP are secreted at low basal levels into trabecular culture medium; their secretion levels are increased several-fold by treatment of the cultures with the phorbol mitogen, 12-O-tetradecanoylphorbol-13-acetate (TPA). Characteristics of the trabecular matrix metalloproteinases and TIMP are similar to those secreted by numerous other tissues, including the retinal pigment epithelium. These proteinases may serve an important role in the maintenance and regulation of the trabecular extracellular matrix and, subsequently, of the aqueous humor outflow pathway in normal and glaucomatous eyes.

Original languageEnglish (US)
Pages (from-to)172-180
Number of pages9
JournalInvestigative Ophthalmology and Visual Science
Volume32
Issue number1
StatePublished - 1991

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Trabecular Meshwork
Tissue Inhibitor of Metalloproteinases
Matrix Metalloproteinase Inhibitors
Gelatinases
Peptide Hydrolases
Extracellular Matrix
Polyacrylamide Gel Electrophoresis
Sodium
Matrix Metalloproteinase 3
Matrix Metalloproteinase 1
Retinal Pigment Epithelium
Aqueous Humor
Tetradecanoylphorbol Acetate
Collagenases
Matrix Metalloproteinases
Mitogens
Culture Media
Electrophoresis
Western Blotting
Gels

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Expression of matrix metalloproteinases and inhibitor by human trabecular meshwork. / Alexander, J. P.; Samples, J. R.; Van Buskirk, E. M.; Acott, Ted.

In: Investigative Ophthalmology and Visual Science, Vol. 32, No. 1, 1991, p. 172-180.

Research output: Contribution to journalArticle

Alexander, J. P. ; Samples, J. R. ; Van Buskirk, E. M. ; Acott, Ted. / Expression of matrix metalloproteinases and inhibitor by human trabecular meshwork. In: Investigative Ophthalmology and Visual Science. 1991 ; Vol. 32, No. 1. pp. 172-180.
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