Expression of lynx1 in developing lung and its modulation by prenatal nicotine exposure

Harmanjatinder S. Sekhon, Pingfang Song, Yibing Jia, Jon Lindstrom, Eliot Spindel

    Research output: Contribution to journalArticle

    31 Citations (Scopus)

    Abstract

    The expression of nicotinic acetylcholine receptors (nAChR) in fetal lung suggests maternal smoking during pregnancy effects newborn lung structure and function by the direct interaction of nicotine with nAChR in the developing lung. The recent identification of the lynx1 nAChR modulator protein in nicotinic neurons in the brain suggests that lynx1 may be similarly expressed in the lung. To study this, cDNAs encoding lynx1 were cloned from rhesus monkey lung. The temporal expression of lynx1 was studied in pre- and postnatal monkey lungs by in situ hybridization, immunohistochemistry, and realtime polymerase chain reaction (PCR). Lynx1 mRNA signal and lynx1 immunohistochemical staining were localized predominantly in airway epithelial cells, submucous glands, and smooth muscle cells, in endothelial and smooth muscle cells in vessel walls, and in alveolar type II cells. The distribution of lynx1 was similar to that of α4, β2, and β4 nAChR expression as determined by immunohistochemistry. Immunohistochemical staining also co-localized choline acetyltransferase, the enzyme that synthesizes acetylcholine, with lynx1 expression. Lynx1 expression was first observed in 71-day fetal lungs and increased with age. Immunohistochemistry, Western analysis, and realtime PCR analysis showed increased lynx1 expression in lungs following prenatal nicotine exposure. Thus, lynx1 is co-expressed with nAChR in the lung. Alteration of lynx1 levels is a potential new mechanism by which nicotine affects lung development.

    Original languageEnglish (US)
    Pages (from-to)287-297
    Number of pages11
    JournalCell and Tissue Research
    Volume320
    Issue number2
    DOIs
    StatePublished - May 2005

    Fingerprint

    Nicotinic Receptors
    Nicotine
    Modulation
    Lung
    Polymerase chain reaction
    Muscle
    Cells
    Choline O-Acetyltransferase
    Immunohistochemistry
    Modulators
    Acetylcholine
    Neurons
    Smooth Muscle Myocytes
    Brain
    Complementary DNA
    Staining and Labeling
    Alveolar Epithelial Cells
    Polymerase Chain Reaction
    Messenger RNA
    Enzymes

    Keywords

    • Acetylcholine
    • Bronchial epithelium
    • Fetal rhesus monkey
    • Lung
    • Ly-6
    • Nicotinic receptor

    ASJC Scopus subject areas

    • Anatomy
    • Clinical Biochemistry
    • Cell Biology

    Cite this

    Expression of lynx1 in developing lung and its modulation by prenatal nicotine exposure. / Sekhon, Harmanjatinder S.; Song, Pingfang; Jia, Yibing; Lindstrom, Jon; Spindel, Eliot.

    In: Cell and Tissue Research, Vol. 320, No. 2, 05.2005, p. 287-297.

    Research output: Contribution to journalArticle

    Sekhon, Harmanjatinder S. ; Song, Pingfang ; Jia, Yibing ; Lindstrom, Jon ; Spindel, Eliot. / Expression of lynx1 in developing lung and its modulation by prenatal nicotine exposure. In: Cell and Tissue Research. 2005 ; Vol. 320, No. 2. pp. 287-297.
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