Expression of insulin-like growth factor-I during chicken development

Kiyoshi Kikuchi, Frances C. Buonomo, Yoshitaka Kajimoto, Peter Rotwein

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

We have investigated the expression of insulin-like growth factor-I (IGF-I) during ontogeny in the chick. IGF-I mRNA was first detectable in whole embryos on day 6, while serum IGF-I could be measured on day 9, the earliest time point examined. Serum IGF-I values rose 10-fold from about 3 ng/ml on day 10 to a prehatch peak of 30-35 ng/ml during days 15-17, and then declined to about 10 ng/ml at the time of hatching. On days 17 and 20 of incubation, IGF-I mRNA was detected in eye, skeletal muscle, and brain, but could not be found in liver or heart until after hatching. During the posthatch period, serum IGF-I rose from 10 ng/ml in the first week to 35-40 ng/ml during weeks 3-6, and liver IGF-I mRNA increased nearly 5-fold from weeks 1-7. The increases observed during weeks 1-3 correlated with a posthatch rise in serum GH from 21 to 37 ng/ml, although GH levels declined over the subsequent 4 weeks without an appreciable change in serum IGF-I values. By contrast, before embryonic day 12 no GH could be detected in the circulation, while IGF-I was 19 ng/ml on day 11. These observations suggest that there is both GH-dependent and GH-independent regulation of IGF-I gene expression in the chick, as has been found in mammals, and support the idea that IGF-I plays a role in chicken embryonic development.

Original languageEnglish (US)
Pages (from-to)1323-1328
Number of pages6
JournalEndocrinology
Volume128
Issue number3
StatePublished - Mar 1991
Externally publishedYes

Fingerprint

Insulin-Like Growth Factor I
Chickens
Serum
Messenger RNA
Liver
Embryonic Development
Mammals
Skeletal Muscle
Embryonic Structures
Gene Expression
Brain

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Kikuchi, K., Buonomo, F. C., Kajimoto, Y., & Rotwein, P. (1991). Expression of insulin-like growth factor-I during chicken development. Endocrinology, 128(3), 1323-1328.

Expression of insulin-like growth factor-I during chicken development. / Kikuchi, Kiyoshi; Buonomo, Frances C.; Kajimoto, Yoshitaka; Rotwein, Peter.

In: Endocrinology, Vol. 128, No. 3, 03.1991, p. 1323-1328.

Research output: Contribution to journalArticle

Kikuchi, K, Buonomo, FC, Kajimoto, Y & Rotwein, P 1991, 'Expression of insulin-like growth factor-I during chicken development', Endocrinology, vol. 128, no. 3, pp. 1323-1328.
Kikuchi K, Buonomo FC, Kajimoto Y, Rotwein P. Expression of insulin-like growth factor-I during chicken development. Endocrinology. 1991 Mar;128(3):1323-1328.
Kikuchi, Kiyoshi ; Buonomo, Frances C. ; Kajimoto, Yoshitaka ; Rotwein, Peter. / Expression of insulin-like growth factor-I during chicken development. In: Endocrinology. 1991 ; Vol. 128, No. 3. pp. 1323-1328.
@article{6c0325a008644efb9b18e89e11e1d3de,
title = "Expression of insulin-like growth factor-I during chicken development",
abstract = "We have investigated the expression of insulin-like growth factor-I (IGF-I) during ontogeny in the chick. IGF-I mRNA was first detectable in whole embryos on day 6, while serum IGF-I could be measured on day 9, the earliest time point examined. Serum IGF-I values rose 10-fold from about 3 ng/ml on day 10 to a prehatch peak of 30-35 ng/ml during days 15-17, and then declined to about 10 ng/ml at the time of hatching. On days 17 and 20 of incubation, IGF-I mRNA was detected in eye, skeletal muscle, and brain, but could not be found in liver or heart until after hatching. During the posthatch period, serum IGF-I rose from 10 ng/ml in the first week to 35-40 ng/ml during weeks 3-6, and liver IGF-I mRNA increased nearly 5-fold from weeks 1-7. The increases observed during weeks 1-3 correlated with a posthatch rise in serum GH from 21 to 37 ng/ml, although GH levels declined over the subsequent 4 weeks without an appreciable change in serum IGF-I values. By contrast, before embryonic day 12 no GH could be detected in the circulation, while IGF-I was 19 ng/ml on day 11. These observations suggest that there is both GH-dependent and GH-independent regulation of IGF-I gene expression in the chick, as has been found in mammals, and support the idea that IGF-I plays a role in chicken embryonic development.",
author = "Kiyoshi Kikuchi and Buonomo, {Frances C.} and Yoshitaka Kajimoto and Peter Rotwein",
year = "1991",
month = "3",
language = "English (US)",
volume = "128",
pages = "1323--1328",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "3",

}

TY - JOUR

T1 - Expression of insulin-like growth factor-I during chicken development

AU - Kikuchi, Kiyoshi

AU - Buonomo, Frances C.

AU - Kajimoto, Yoshitaka

AU - Rotwein, Peter

PY - 1991/3

Y1 - 1991/3

N2 - We have investigated the expression of insulin-like growth factor-I (IGF-I) during ontogeny in the chick. IGF-I mRNA was first detectable in whole embryos on day 6, while serum IGF-I could be measured on day 9, the earliest time point examined. Serum IGF-I values rose 10-fold from about 3 ng/ml on day 10 to a prehatch peak of 30-35 ng/ml during days 15-17, and then declined to about 10 ng/ml at the time of hatching. On days 17 and 20 of incubation, IGF-I mRNA was detected in eye, skeletal muscle, and brain, but could not be found in liver or heart until after hatching. During the posthatch period, serum IGF-I rose from 10 ng/ml in the first week to 35-40 ng/ml during weeks 3-6, and liver IGF-I mRNA increased nearly 5-fold from weeks 1-7. The increases observed during weeks 1-3 correlated with a posthatch rise in serum GH from 21 to 37 ng/ml, although GH levels declined over the subsequent 4 weeks without an appreciable change in serum IGF-I values. By contrast, before embryonic day 12 no GH could be detected in the circulation, while IGF-I was 19 ng/ml on day 11. These observations suggest that there is both GH-dependent and GH-independent regulation of IGF-I gene expression in the chick, as has been found in mammals, and support the idea that IGF-I plays a role in chicken embryonic development.

AB - We have investigated the expression of insulin-like growth factor-I (IGF-I) during ontogeny in the chick. IGF-I mRNA was first detectable in whole embryos on day 6, while serum IGF-I could be measured on day 9, the earliest time point examined. Serum IGF-I values rose 10-fold from about 3 ng/ml on day 10 to a prehatch peak of 30-35 ng/ml during days 15-17, and then declined to about 10 ng/ml at the time of hatching. On days 17 and 20 of incubation, IGF-I mRNA was detected in eye, skeletal muscle, and brain, but could not be found in liver or heart until after hatching. During the posthatch period, serum IGF-I rose from 10 ng/ml in the first week to 35-40 ng/ml during weeks 3-6, and liver IGF-I mRNA increased nearly 5-fold from weeks 1-7. The increases observed during weeks 1-3 correlated with a posthatch rise in serum GH from 21 to 37 ng/ml, although GH levels declined over the subsequent 4 weeks without an appreciable change in serum IGF-I values. By contrast, before embryonic day 12 no GH could be detected in the circulation, while IGF-I was 19 ng/ml on day 11. These observations suggest that there is both GH-dependent and GH-independent regulation of IGF-I gene expression in the chick, as has been found in mammals, and support the idea that IGF-I plays a role in chicken embryonic development.

UR - http://www.scopus.com/inward/record.url?scp=0025763306&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025763306&partnerID=8YFLogxK

M3 - Article

VL - 128

SP - 1323

EP - 1328

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 3

ER -