Expression of extraocular-superfast-myosin heavy chain in rat laryngeal muscles

Akihiro Shiotani, Paul Flint

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Superfast myosin heavy chain (MHC), which is found in jaw-closing muscle and extraocular muscle (EOM), may also be found in rat laryngeal muscles. Immunostaining and Western blot using anti-EOM antibody were performed to identify and localize EOM MHC in laryngeal muscles. Specific reactivity of laryngeal IIL MHC was confirmed by Western blot and on immunostaining, all fibers in the lateral part of thyroarytenoid muscle reacted with EOM antibody. A scattered pattern of positive fibers was observed in the medial part of the thyroarytenoid, the posterior cricoarytenoid and the lateral cricoarytenoid muscles. EOM MHC was not detected in the cricothyroid muscle. The expression of EOM MHC in rat laryngeal muscle is consistent with the functional demands of the airway protection reflex.

Original languageEnglish (US)
Pages (from-to)3639-3642
Number of pages4
JournalNeuroReport
Volume9
Issue number16
StatePublished - Nov 16 1998
Externally publishedYes

Fingerprint

Oculomotor Muscles
Laryngeal Muscles
Myosin Heavy Chains
Western Blotting
Antibodies
Jaw
Reflex
Muscles

Keywords

  • Extraocular muscle
  • Immunohistochemistry
  • Myosin heavy chain
  • Rat laryngeal muscle
  • SDS-PAGE
  • Thyroarytenoid muscle
  • Western blot

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Expression of extraocular-superfast-myosin heavy chain in rat laryngeal muscles. / Shiotani, Akihiro; Flint, Paul.

In: NeuroReport, Vol. 9, No. 16, 16.11.1998, p. 3639-3642.

Research output: Contribution to journalArticle

@article{5d603d48b25f4e2dacc07cf4f695b6f5,
title = "Expression of extraocular-superfast-myosin heavy chain in rat laryngeal muscles",
abstract = "Superfast myosin heavy chain (MHC), which is found in jaw-closing muscle and extraocular muscle (EOM), may also be found in rat laryngeal muscles. Immunostaining and Western blot using anti-EOM antibody were performed to identify and localize EOM MHC in laryngeal muscles. Specific reactivity of laryngeal IIL MHC was confirmed by Western blot and on immunostaining, all fibers in the lateral part of thyroarytenoid muscle reacted with EOM antibody. A scattered pattern of positive fibers was observed in the medial part of the thyroarytenoid, the posterior cricoarytenoid and the lateral cricoarytenoid muscles. EOM MHC was not detected in the cricothyroid muscle. The expression of EOM MHC in rat laryngeal muscle is consistent with the functional demands of the airway protection reflex.",
keywords = "Extraocular muscle, Immunohistochemistry, Myosin heavy chain, Rat laryngeal muscle, SDS-PAGE, Thyroarytenoid muscle, Western blot",
author = "Akihiro Shiotani and Paul Flint",
year = "1998",
month = "11",
day = "16",
language = "English (US)",
volume = "9",
pages = "3639--3642",
journal = "NeuroReport",
issn = "0959-4965",
publisher = "Lippincott Williams and Wilkins",
number = "16",

}

TY - JOUR

T1 - Expression of extraocular-superfast-myosin heavy chain in rat laryngeal muscles

AU - Shiotani, Akihiro

AU - Flint, Paul

PY - 1998/11/16

Y1 - 1998/11/16

N2 - Superfast myosin heavy chain (MHC), which is found in jaw-closing muscle and extraocular muscle (EOM), may also be found in rat laryngeal muscles. Immunostaining and Western blot using anti-EOM antibody were performed to identify and localize EOM MHC in laryngeal muscles. Specific reactivity of laryngeal IIL MHC was confirmed by Western blot and on immunostaining, all fibers in the lateral part of thyroarytenoid muscle reacted with EOM antibody. A scattered pattern of positive fibers was observed in the medial part of the thyroarytenoid, the posterior cricoarytenoid and the lateral cricoarytenoid muscles. EOM MHC was not detected in the cricothyroid muscle. The expression of EOM MHC in rat laryngeal muscle is consistent with the functional demands of the airway protection reflex.

AB - Superfast myosin heavy chain (MHC), which is found in jaw-closing muscle and extraocular muscle (EOM), may also be found in rat laryngeal muscles. Immunostaining and Western blot using anti-EOM antibody were performed to identify and localize EOM MHC in laryngeal muscles. Specific reactivity of laryngeal IIL MHC was confirmed by Western blot and on immunostaining, all fibers in the lateral part of thyroarytenoid muscle reacted with EOM antibody. A scattered pattern of positive fibers was observed in the medial part of the thyroarytenoid, the posterior cricoarytenoid and the lateral cricoarytenoid muscles. EOM MHC was not detected in the cricothyroid muscle. The expression of EOM MHC in rat laryngeal muscle is consistent with the functional demands of the airway protection reflex.

KW - Extraocular muscle

KW - Immunohistochemistry

KW - Myosin heavy chain

KW - Rat laryngeal muscle

KW - SDS-PAGE

KW - Thyroarytenoid muscle

KW - Western blot

UR - http://www.scopus.com/inward/record.url?scp=0032538864&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032538864&partnerID=8YFLogxK

M3 - Article

C2 - 9858372

AN - SCOPUS:0032538864

VL - 9

SP - 3639

EP - 3642

JO - NeuroReport

JF - NeuroReport

SN - 0959-4965

IS - 16

ER -