Expression of apoptosis-related genes in chronic cyclosporine nephrotoxicity in mice

Chul Woo Yang, Gregory R. Faulkner, Ihab M. Wahba, Tracy A. Christianson, Grover C. Bagby, Dong Chan Jin, Hanna E. Abboud, Takeshi F. Andoh, William M. Bennett

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    Abstract

    To define the mechanism of cyclosporine (CsA)-induced apoptosis, we investigated the expression of apoptosis-related genes in experimental chronic CsA nephrotoxicity. Mice on a low-salt (0.01%) diet were given vehicle (VH, olive oil, 1 mg/kg/day), or CsA (30mg/kg/day), and sacrificed at 1 and 4weeks. Apoptosis was detected with deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) stain, and the expressions of apoptosis-related genes were evaluated by reverse transcription-polymerase chain reaction, immunoblot or immunohistochemistry. The activity of caspase 1 and 3 was also evaluated. The CsA group showed increases in apoptotic cells compared with the VH group (54±41 vs. 3±3, p

    Original languageEnglish (US)
    Pages (from-to)391-399
    Number of pages9
    JournalAmerican Journal of Transplantation
    Volume2
    Issue number5
    DOIs
    Publication statusPublished - May 2002

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    Keywords

    • Apoptosis
    • Cyclosporine
    • Mice

    ASJC Scopus subject areas

    • Immunology

    Cite this

    Yang, C. W., Faulkner, G. R., Wahba, I. M., Christianson, T. A., Bagby, G. C., Jin, D. C., ... Bennett, W. M. (2002). Expression of apoptosis-related genes in chronic cyclosporine nephrotoxicity in mice. American Journal of Transplantation, 2(5), 391-399. https://doi.org/10.1034/j.1600-6143.2002.20501.x