Expression of a novel high-affinity purine nucleobase transport function in mutant mammalian T lymphoblasts.

B. Aronow, D. Toll, J. Patrick, P. Hollingsworth, K. McCartan, Buddy Ullman

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The single nucleoside transport function of mouse S49 lymphoblasts also transports purine bases (B. Aronow and B. Ullman, J. Biol. Chem. 261:2014-2019, 1986). This transport of purine bases by S49 cells is sensitive to inhibition by dipyridamole (DPA) and 4-nitrobenzylthioinosine, two potent inhibitors of nucleoside transport. Therefore, wild-type S49 cells cannot salvage low hypoxanthine concentrations in the presence of 10 microM DPA and 11 microM azaserine; the latter is a potent inhibitor of purine biosynthesis. Among a mutagenized wild-type population, a cell line, JPA2, was isolated which could proliferate in 50 microM hypoxanthine-11 microM azaserine-10 microM DPA. The basis for the survival of JPA2 cells under these selective conditions was expression of a unique, high-affinity purine nucleobase transport function not present in wild-type cells. JPA2 cells could transport 5 microM concentrations of hypoxanthine, guanine, and adenine 15- to 30-fold more efficiently than parental cells did. Kinetic analyses revealed that the affinity of the JPA2 transporter for all three purine bases was much greater than that of the wild-type nucleobase transport system. Moreover, nucleobase transport in JPA2 cells, unlike that in parental cells, was insensitive to inhibition by DPA, 4-nitrobenzylthioinosine, sulfhydryl reagents, and nucleosides. No alterations in nucleoside transport capability, phosphoribosylpyrophosphate levels, or purine phosphoribosyltransferase enzymes were detected in JPA2 cells. Thus, JPA2 cells express a novel nucleobase transport capability which can be distinguished from the nucleoside transport function by multiple biochemical parameters.

Original languageEnglish (US)
Pages (from-to)2957-2962
Number of pages6
JournalMolecular and Cellular Biology
Volume6
Issue number8
StatePublished - Aug 1986
Externally publishedYes

Fingerprint

Nucleosides
Dipyridamole
Hypoxanthine
Azaserine
purine
Sulfhydryl Reagents
Guanine
Adenine
Cell Survival
Cell Line
Enzymes
Population
4-nitrobenzylthioinosine

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Aronow, B., Toll, D., Patrick, J., Hollingsworth, P., McCartan, K., & Ullman, B. (1986). Expression of a novel high-affinity purine nucleobase transport function in mutant mammalian T lymphoblasts. Molecular and Cellular Biology, 6(8), 2957-2962.

Expression of a novel high-affinity purine nucleobase transport function in mutant mammalian T lymphoblasts. / Aronow, B.; Toll, D.; Patrick, J.; Hollingsworth, P.; McCartan, K.; Ullman, Buddy.

In: Molecular and Cellular Biology, Vol. 6, No. 8, 08.1986, p. 2957-2962.

Research output: Contribution to journalArticle

Aronow, B, Toll, D, Patrick, J, Hollingsworth, P, McCartan, K & Ullman, B 1986, 'Expression of a novel high-affinity purine nucleobase transport function in mutant mammalian T lymphoblasts.', Molecular and Cellular Biology, vol. 6, no. 8, pp. 2957-2962.
Aronow B, Toll D, Patrick J, Hollingsworth P, McCartan K, Ullman B. Expression of a novel high-affinity purine nucleobase transport function in mutant mammalian T lymphoblasts. Molecular and Cellular Biology. 1986 Aug;6(8):2957-2962.
Aronow, B. ; Toll, D. ; Patrick, J. ; Hollingsworth, P. ; McCartan, K. ; Ullman, Buddy. / Expression of a novel high-affinity purine nucleobase transport function in mutant mammalian T lymphoblasts. In: Molecular and Cellular Biology. 1986 ; Vol. 6, No. 8. pp. 2957-2962.
@article{4f8bf86acb1446b3842360436b882da1,
title = "Expression of a novel high-affinity purine nucleobase transport function in mutant mammalian T lymphoblasts.",
abstract = "The single nucleoside transport function of mouse S49 lymphoblasts also transports purine bases (B. Aronow and B. Ullman, J. Biol. Chem. 261:2014-2019, 1986). This transport of purine bases by S49 cells is sensitive to inhibition by dipyridamole (DPA) and 4-nitrobenzylthioinosine, two potent inhibitors of nucleoside transport. Therefore, wild-type S49 cells cannot salvage low hypoxanthine concentrations in the presence of 10 microM DPA and 11 microM azaserine; the latter is a potent inhibitor of purine biosynthesis. Among a mutagenized wild-type population, a cell line, JPA2, was isolated which could proliferate in 50 microM hypoxanthine-11 microM azaserine-10 microM DPA. The basis for the survival of JPA2 cells under these selective conditions was expression of a unique, high-affinity purine nucleobase transport function not present in wild-type cells. JPA2 cells could transport 5 microM concentrations of hypoxanthine, guanine, and adenine 15- to 30-fold more efficiently than parental cells did. Kinetic analyses revealed that the affinity of the JPA2 transporter for all three purine bases was much greater than that of the wild-type nucleobase transport system. Moreover, nucleobase transport in JPA2 cells, unlike that in parental cells, was insensitive to inhibition by DPA, 4-nitrobenzylthioinosine, sulfhydryl reagents, and nucleosides. No alterations in nucleoside transport capability, phosphoribosylpyrophosphate levels, or purine phosphoribosyltransferase enzymes were detected in JPA2 cells. Thus, JPA2 cells express a novel nucleobase transport capability which can be distinguished from the nucleoside transport function by multiple biochemical parameters.",
author = "B. Aronow and D. Toll and J. Patrick and P. Hollingsworth and K. McCartan and Buddy Ullman",
year = "1986",
month = "8",
language = "English (US)",
volume = "6",
pages = "2957--2962",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "8",

}

TY - JOUR

T1 - Expression of a novel high-affinity purine nucleobase transport function in mutant mammalian T lymphoblasts.

AU - Aronow, B.

AU - Toll, D.

AU - Patrick, J.

AU - Hollingsworth, P.

AU - McCartan, K.

AU - Ullman, Buddy

PY - 1986/8

Y1 - 1986/8

N2 - The single nucleoside transport function of mouse S49 lymphoblasts also transports purine bases (B. Aronow and B. Ullman, J. Biol. Chem. 261:2014-2019, 1986). This transport of purine bases by S49 cells is sensitive to inhibition by dipyridamole (DPA) and 4-nitrobenzylthioinosine, two potent inhibitors of nucleoside transport. Therefore, wild-type S49 cells cannot salvage low hypoxanthine concentrations in the presence of 10 microM DPA and 11 microM azaserine; the latter is a potent inhibitor of purine biosynthesis. Among a mutagenized wild-type population, a cell line, JPA2, was isolated which could proliferate in 50 microM hypoxanthine-11 microM azaserine-10 microM DPA. The basis for the survival of JPA2 cells under these selective conditions was expression of a unique, high-affinity purine nucleobase transport function not present in wild-type cells. JPA2 cells could transport 5 microM concentrations of hypoxanthine, guanine, and adenine 15- to 30-fold more efficiently than parental cells did. Kinetic analyses revealed that the affinity of the JPA2 transporter for all three purine bases was much greater than that of the wild-type nucleobase transport system. Moreover, nucleobase transport in JPA2 cells, unlike that in parental cells, was insensitive to inhibition by DPA, 4-nitrobenzylthioinosine, sulfhydryl reagents, and nucleosides. No alterations in nucleoside transport capability, phosphoribosylpyrophosphate levels, or purine phosphoribosyltransferase enzymes were detected in JPA2 cells. Thus, JPA2 cells express a novel nucleobase transport capability which can be distinguished from the nucleoside transport function by multiple biochemical parameters.

AB - The single nucleoside transport function of mouse S49 lymphoblasts also transports purine bases (B. Aronow and B. Ullman, J. Biol. Chem. 261:2014-2019, 1986). This transport of purine bases by S49 cells is sensitive to inhibition by dipyridamole (DPA) and 4-nitrobenzylthioinosine, two potent inhibitors of nucleoside transport. Therefore, wild-type S49 cells cannot salvage low hypoxanthine concentrations in the presence of 10 microM DPA and 11 microM azaserine; the latter is a potent inhibitor of purine biosynthesis. Among a mutagenized wild-type population, a cell line, JPA2, was isolated which could proliferate in 50 microM hypoxanthine-11 microM azaserine-10 microM DPA. The basis for the survival of JPA2 cells under these selective conditions was expression of a unique, high-affinity purine nucleobase transport function not present in wild-type cells. JPA2 cells could transport 5 microM concentrations of hypoxanthine, guanine, and adenine 15- to 30-fold more efficiently than parental cells did. Kinetic analyses revealed that the affinity of the JPA2 transporter for all three purine bases was much greater than that of the wild-type nucleobase transport system. Moreover, nucleobase transport in JPA2 cells, unlike that in parental cells, was insensitive to inhibition by DPA, 4-nitrobenzylthioinosine, sulfhydryl reagents, and nucleosides. No alterations in nucleoside transport capability, phosphoribosylpyrophosphate levels, or purine phosphoribosyltransferase enzymes were detected in JPA2 cells. Thus, JPA2 cells express a novel nucleobase transport capability which can be distinguished from the nucleoside transport function by multiple biochemical parameters.

UR - http://www.scopus.com/inward/record.url?scp=0022766099&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022766099&partnerID=8YFLogxK

M3 - Article

C2 - 3491294

AN - SCOPUS:0022766099

VL - 6

SP - 2957

EP - 2962

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 8

ER -